Reti L L, Beck F, Bulman S
J Exp Zool. 1982 Oct 10;223(2):197-9. doi: 10.1002/jez.1402230214.
We attempted to grow explanted 91/2-day rat embryos in vitro in human serum but were unable to demonstrate normal growth as has been previously described. The embryos were consistently smaller, anaemic, and frequently deformed. These abnormalities were overcome by supplementing the human serum with 10% rat serum. The latter possibly provides yet unidentified essential growth factors required by rat embryos that are not present in human serum.
我们试图在人血清中对9.5日龄的大鼠胚胎进行体外培养，但未能像之前所描述的那样实现正常生长。这些胚胎始终较小、贫血，且经常出现畸形。通过在人血清中添加10%的大鼠血清，这些异常情况得到了克服。大鼠血清可能提供了人血清中不存在但大鼠胚胎所需的尚未明确的必需生长因子。
