State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, 575 W Stadium Ave, West Lafayette, IN 47907, USA.
Nutrients. 2021 Dec 12;13(12):4441. doi: 10.3390/nu13124441.
Acute liver injury (ALI) has a high mortality rate of approximately 20-40%, and it is imperative to find complementary and alternative drugs for treating ALI. A carbon tetrachloride (CCl)-induced ALI mouse model was established to explore whether dietary intervention can alleviate ALI in mice. Intestinal flora, intestinal integrity, biomarkers of hepatic function, systemic inflammation, autophagy, and apoptosis signals were detected through a real-time PCR, hematoxylin-eosin staining, 16S rRNA gene sequencing, and so on. The results showed that 1201 had a strongly antioxidant ability, and galactooligosaccharide (GOS) could boost its growth. Based on these findings, the combination of 1201 and GOS, the synbiotic, was applied to prevent CCl-induced ALI in mice. The current research proved that GOS promoted the intestinal colonization of 1201, and the synbiotic improved the antioxidant capacity of the host, regulated the intestinal flora, repaired the intestinal barrier, inhibited the activation of the MAPK/NF-κB pathway, and then inhibited the apoptosis and autophagy pathways, relieving inflammation and liver oxidation; thereby, the ALI of mice was alleviated. These results suggest that synbiotics may become a new research direction for liver-protecting drugs.
急性肝损伤（ALI）的死亡率约为 20-40%，因此寻找治疗 ALI 的补充和替代药物势在必行。本研究建立了四氯化碳（CCl）诱导的 ALI 小鼠模型，以探讨饮食干预是否能减轻小鼠的 ALI。通过实时 PCR、苏木精-伊红染色、16S rRNA 基因测序等方法检测了肠道菌群、肠道完整性、肝功能标志物、全身炎症、自噬和细胞凋亡信号。结果表明，1201 具有很强的抗氧化能力，半乳糖低聚糖（GOS）可以促进其生长。基于这些发现，将 1201 和 GOS 联合应用于预防 CCl 诱导的 ALI 小鼠。本研究证明 GOS 促进了 1201 在肠道中的定植，共生元提高了宿主的抗氧化能力，调节了肠道菌群，修复了肠道屏障，抑制了 MAPK/NF-κB 通路的激活，从而抑制了细胞凋亡和自噬通路，缓解了炎症和肝脏氧化；从而减轻了小鼠的 ALI。这些结果表明，共生元可能成为一种新的护肝药物研究方向。
