Department of Oncology of Integrative Chinese and Western Medicine, China-Japan Friendship Hospital, Beijing 100029, China ; School of Clinical Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Evid Based Complement Alternat Med. 2012;2012:381904. doi: 10.1155/2012/381904. Epub 2012 Nov 6.
Bone metastasis (BM) is a major clinical problem for which current treatments lack full efficacy. The Traditional Chinese Medicine (TCM) Sangu Decoction (SGD) has been widely used to treat BM in China. However, no in vivo experiments to date have investigated the effects of TCM on osteoclast activity in BM. In this study, the protective effect and probable mechanism of SGD were evaluated. The model was established using the breast cancer MRMT-1 cells injected into the tibia of rat. SGD was administrated, compared with Zoledronic acid as a positive control. The development of the bone tumor and osteoclast activity was monitored by radiological analysis. TRAP stain was used to identify osteoclasts quantity and activity. TRAP-5b in serum or bone tumor and TRAP mRNA were also quantified. Radiological examination showed that SGD inhibited tumor proliferation and preserved the cortical and trabecular bone structure. In addition, a dramatic reduction of TRAP positive osteoclasts was observed and TRAP-5b levels in serum and bone tumor decreased significantly. It also reduced the mRNA expression of TRAP. The results indicated that SGD exerted potent antiosteoclast property that could be directly related to its TRAP inhibited activity. In addition it prevented bone tumor proliferation in BM model.
骨转移（BM）是一个主要的临床问题，目前的治疗方法缺乏完全的疗效。中药三骨汤（SGD）已在中国广泛用于治疗 BM。然而，迄今为止，没有体内实验研究过中药对 BM 中破骨细胞活性的影响。在这项研究中，评估了 SGD 的保护作用和可能的机制。使用乳腺癌 MRMT-1 细胞注射到大鼠胫骨中建立模型。给予 SGD，与唑来膦酸作为阳性对照进行比较。通过放射学分析监测骨肿瘤的发展和破骨细胞活性。TRAP 染色用于鉴定破骨细胞的数量和活性。还定量了血清或骨肿瘤中的 TRAP-5b 和 TRAP mRNA。放射学检查显示 SGD 抑制了肿瘤的增殖并保持了皮质骨和小梁骨的结构。此外，观察到 TRAP 阳性破骨细胞数量明显减少，血清和骨肿瘤中的 TRAP-5b 水平显著降低。它还降低了 TRAP 的 mRNA 表达。结果表明，SGD 具有强大的抗破骨细胞特性，这可能与其 TRAP 抑制活性直接相关。此外，它还可以防止 BM 模型中的骨肿瘤增殖。
