Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, People's Republic of China.
Diagn Pathol. 2012 Dec 17;7:180. doi: 10.1186/1746-1596-7-180.
ATP-binding cassette sub-family G member 2 (ABCG2) is a protein that in humans is encoded by the ABCG2 gene. ABCG2 participates in efflux of many chemotherapeutic agents. ABCG2 is often expressed in hematopoietic progenitor or stem cells. Vacuolar-H + -ATPase (V-ATPase) plays a key role in adjusting and maintaining intracellular pH and in regulating the drug tolerance of cells. The TNM Classification of Malignant Tumours (TNM) is a cancer staging system that describes the extent of cancer in a patient's body. In this study, the expression of ABCG2 and V-ATPase in esophageal squamous cancer cells was detected.
Immunohistochemistry staining and Immunofluorescence double staining were used to detect the expression of ABCG2 and V-ATPase in in 66 cases of esophageal squamous cancer cells. Associations and differences in expression of ABCG2 with that of V-ATPase were analyzed.
Positive staining patterns for both ABCG2 (66.67%) and V-ATPase (68.18%) were located mainly in the plasma membrane and cytoplasm. Marked differences in expression were also shown (P < 0.001) among 3 groups of pathological grades and TNM stages in these carcinomas. Marked differences were also found for ABCG2 expression between the two groups in the pathological grades and in the TNM staging groups (P < 0.01), but not between the αb and βgroups. V-ATPase expression was statistically significant between the 2 groups in the pathological grades and TNM stages (P < 0.05). This was not evident between α and β groups of pathological grades or between αb and βof the TNM stages. Marked differences in expression of ABCG2 and V-ATPase were found between metastatic and non-metastatic groups in the same carcinomas (P < 0.0001). There was also a clear correlation between the expression of ABCG2 and V-ATPase (P ≤ 0.001) in the various groups of pathological grades and TNM stages.
Both ABCG2 and V-ATPase were over-expressed in esophageal squamous cancer cells. Their expression was associated with pathological grade, TNM stage and tumor metastasis in esophageal squamous cancer cells, suggesting interaction relationship between them. ABCG2 and V-ATPase expression may be strongly associated with drug resistance and tumor metastasis.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/3823783918433897.
三磷酸腺苷结合盒亚家族 G 成员 2（ABCG2）是一种蛋白质，在人类中由 ABCG2 基因编码。ABCG2 参与许多化疗药物的外排。ABCG2 通常在造血祖细胞或干细胞中表达。液泡型 H + -ATP 酶（V-ATPase）在调节和维持细胞内 pH 值以及调节细胞的药物耐受性方面发挥着关键作用。恶性肿瘤的 TNM 分类（TNM）是一种描述患者体内癌症程度的癌症分期系统。在这项研究中，检测了食管鳞状癌细胞中 ABCG2 和 V-ATPase 的表达。
使用免疫组织化学染色和免疫荧光双重染色检测 66 例食管鳞状癌细胞中 ABCG2 和 V-ATPase 的表达。分析 ABCG2 与 V-ATPase 表达的相关性和差异。
ABCG2（66.67%）和 V-ATPase（68.18%）的阳性染色模式主要位于质膜和细胞质中。在这些癌中，3 组病理分级和 TNM 分期之间也显示出明显的差异（P<0.001）。在病理分级和 TNM 分期组中，ABCG2 表达在两组之间也存在显著差异（P<0.01），但在 αb 和 β 组之间没有差异。V-ATPase 表达在病理分级和 TNM 分期两组之间有统计学意义（P<0.05）。在病理分级的 α 和 β 组之间以及 TNM 分期的 αb 和 β 组之间没有明显差异。在同一癌中，转移组和非转移组之间的 ABCG2 和 V-ATPase 表达差异明显（P<0.0001）。在各种病理分级和 TNM 分期组中，ABCG2 和 V-ATPase 的表达之间也存在明显的相关性（P≤0.001）。
食管鳞状癌细胞中 ABCG2 和 V-ATPase 均过度表达。它们的表达与食管鳞状癌细胞的病理分级、TNM 分期和肿瘤转移有关，提示它们之间存在相互作用关系。ABCG2 和 V-ATPase 的表达可能与药物耐药性和肿瘤转移密切相关。
