Department of Biological Sciences and Center for Structural Biology, Vanderbilt University, Nashville, TN 37235, USA.
Virology. 2013 Feb 5;436(1):173-8. doi: 10.1016/j.virol.2012.11.008. Epub 2012 Dec 11.
We have used fiber diffraction, cryo-electron microscopy, and scanning transmission electron microscopy to confirm the symmetry of three potexviruses, potato virus X, papaya mosaic virus, and narcissus mosaic virus, and to determine their low-resolution structures. All three viruses have slightly less than nine subunits per turn of the viral helix. Our data strongly support the view that all potexviruses have approximately the same symmetry. The structures are dominated by a large domain at high radius in the virion, with a smaller domain, which includes the putative RNA-binding site, extending to low radius.
我们使用纤维衍射、低温电子显微镜和扫描透射电子显微镜来确认三种马铃薯 Y 病毒属病毒(马铃薯 X 病毒、木瓜花叶病毒和水仙花叶病毒)的对称性,并确定它们的低分辨率结构。这三种病毒的每圈病毒螺旋都略少于 9 个亚基。我们的数据强烈支持所有马铃薯 Y 病毒属病毒具有大致相同的对称性的观点。这些结构主要由病毒粒子中高半径处的一个大结构域主导,一个较小的结构域,包括假定的 RNA 结合位点,延伸到低半径处。