Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Queensland 4072, Australia.
Curr Opin Biotechnol. 2013 Apr;24(2):271-7. doi: 10.1016/j.copbio.2012.08.007. Epub 2012 Sep 1.
Genome-scale metabolic reconstructions are used extensively in the study of microbial metabolism and have proven powerful tools to guide rational pathway design of industrial strains. Generation and curation of plant genome-scale metabolic models has proven far more challenging, not the least of which is our incomplete knowledge of compartmentation and organelle transporters in plants. Conversely, the potential value of modelling is far greater when exploring a complex, multi-organelle and multi-tissue metabolism. The first generation of plant genome-scale metabolic reconstructions have proven surprisingly functional and robust as well as capable of predicting many observed complex phenotypes. With further refinement, the application of these models promises to make important contributions to plant biology and metabolic engineering.
基因组规模的代谢重建被广泛应用于微生物代谢研究，并且已经被证明是指导工业菌株合理途径设计的有力工具。生成和编辑植物基因组规模代谢模型的工作则更具挑战性，这主要是因为我们对植物区室化和细胞器转运蛋白的认识还不完全。相反，在探索复杂的多细胞器和多组织代谢时，建模的潜在价值要大得多。第一代植物基因组规模的代谢重建已经被证明具有惊人的功能和稳健性，并且能够预测许多观察到的复杂表型。随着进一步的改进，这些模型的应用有望为植物生物学和代谢工程做出重要贡献。
