热量限制的 24 月龄大鼠胰岛素刺激的分离骨骼肌中更大的细丝蛋白 C、GSK3α 和 GSK3β 丝氨酸磷酸化。
Greater filamin C, GSK3α, and GSK3β serine phosphorylation in insulin-stimulated isolated skeletal muscles of calorie restricted 24 month-old rats.
机构信息
Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109-2214, USA.
出版信息
Mech Ageing Dev. 2013 Jan-Feb;134(1-2):60-3. doi: 10.1016/j.mad.2012.12.002. Epub 2012 Dec 11.
Abstract
Moderate calorie restriction (CR) can improve insulin-stimulated Akt phosphorylation and glucose uptake in muscles from 24 month-old rats, but the specific Akt substrates linking CR-effects on Akt to glucose uptake and other cellular processes are uncertain. We probed CR's influence on site-specific phosphorylation of five Akt substrates (AS160(Ser588), TBC1D1(Thr596), FLNc(Ser2213), GSK3α(Ser21), and GSK3β(Ser9)) in predominantly fast-twitch (epitrochlearis) and predominantly slow-twitch (soleus) muscles. We observed no CR-effect on phosphorylation of AS160(Ser588) or TBC1D1(Thr596), but there was a CR-induced increase in insulin-stimulated FLNc(Ser2213), GSK3α(Ser21), and GSK3β(Ser9) phosphorylation for both muscles. These results indicate that CR does not uniformly affect insulin-mediated phosphorylation of Akt substrates in fast- or slow-twitch muscles from 24 month-old rats.
摘要
适度热量限制（CR）可以改善 24 月龄大鼠肌肉中胰岛素刺激的 Akt 磷酸化和葡萄糖摄取，但将 CR 对 Akt 的影响与葡萄糖摄取和其他细胞过程联系起来的特定 Akt 底物尚不确定。我们探讨了 CR 对五种 Akt 底物（AS160（Ser588）、TBC1D1（Thr596）、FLNc（Ser2213）、GSK3α（Ser21）和 GSK3β（Ser9））在主要快肌（比目鱼肌）和主要慢肌（跖肌）中特异性磷酸化的影响。我们没有观察到 CR 对 AS160（Ser588）或 TBC1D1（Thr596）磷酸化的影响，但 CR 诱导了胰岛素刺激的 FLNc（Ser2213）、GSK3α（Ser21）和 GSK3β（Ser9）在两种肌肉中的磷酸化增加。这些结果表明，CR 不会均匀影响 24 月龄大鼠快肌或慢肌中胰岛素介导的 Akt 底物磷酸化。
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2
Preventing the calorie restriction-induced increase in insulin-stimulated Akt2 phosphorylation eliminates calorie restriction's effect on glucose uptake in skeletal muscle.阻止热量限制引起的胰岛素刺激的Akt2磷酸化增加，可消除热量限制对骨骼肌葡萄糖摄取的影响。
Biochim Biophys Acta. 2012 Nov;1822(11):1735-40. doi: 10.1016/j.bbadis.2012.07.012. Epub 2012 Jul 27.
3
The Rab-GTPase-activating protein TBC1D1 regulates skeletal muscle glucose metabolism.Rab-GTPase 激活蛋白 TBC1D1 调节骨骼肌葡萄糖代谢。
Am J Physiol Endocrinol Metab. 2012 Aug 15;303(4):E524-33. doi: 10.1152/ajpendo.00605.2011. Epub 2012 Jun 12.
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