Diczfalusy U, Alexson S E
Department of Clinical Chemistry I, Huddinge University Hospital, Sweden.
J Lipid Res. 1990 Feb;31(2):307-14.
We have recently shown that isolated rat liver peroxisomes can chain-shorten prostaglandin F2 alpha and prostaglandin E2 to tetranor-metabolites. In the present report dinor-metabolites of these two prostaglandins were also identified, suggesting that the peroxisomal chain-shortening reaction of prostaglandins is a beta-oxidation reaction. Furthermore, an intermediate containing an extra double bond was isolated from incubates of prostaglandin F2 alpha with peroxisomes. This intermediate was tentatively assigned the structure 2,3-dehydroprostaglandin F2 alpha. Prostaglandin E1 and a major circulating prostaglandin F2 alpha metabolite were also metabolized to chain-shortened products by peroxisomes. The accumulation of the 2,3-dehydro-metabolite and the dinor-metabolites suggest that the peroxisomal beta-oxidation sequence is not tightly coupled, in contrast to mitochondrial fatty acid oxidation.
我们最近发现，分离出的大鼠肝脏过氧化物酶体能够将前列腺素F2α和前列腺素E2链缩短为四降代谢物。在本报告中，还鉴定出了这两种前列腺素的二降代谢物，这表明前列腺素的过氧化物酶体链缩短反应是一种β-氧化反应。此外，从前列腺素F2α与过氧化物酶体的孵育物中分离出了一种含有额外双键的中间体。该中间体暂定为2,3-脱氢前列腺素F2α的结构。前列腺素E1和一种主要的循环前列腺素F2α代谢物也被过氧化物酶体代谢为链缩短产物。2,3-脱氢代谢物和二降代谢物的积累表明，与线粒体脂肪酸氧化相反，过氧化物酶体β-氧化序列的耦合并不紧密。
