Diczfalusy U, Alexson S E, Pedersen J I
Biochem Biophys Res Commun. 1987 May 14;144(3):1206-13. doi: 10.1016/0006-291x(87)91439-2.
Liver peroxisomes were isolated from di(2-ethylhexyl)phthalate treated rats by isopycnic sucrose gradient centrifugation of a light mitochondrial fraction. Incubation of prostaglandin F2 alpha with purified peroxisomes resulted in conversion into a more polar product(s). In contrast, incubation with mitochondrial fractions and microsomal fractions under the same conditions did not result in any detectable conversion. The polar material obtained from a preparative incubation was purified by high performance liquid chromatography and characterized by radio-gas chromatography and gas chromatography-mass spectrometry. The structure of the polar compound was shown to be 5,7,11-trihydroxy-tetranorprost-9-enoic acid (tetranor-prostaglandin F1 alpha). Prostaglandin F2 alpha was thus chain-shortened by four carbon atoms.
通过对轻线粒体部分进行等密度蔗糖梯度离心，从邻苯二甲酸二（2-乙基己基）酯处理的大鼠中分离出肝脏过氧化物酶体。将前列腺素F2α与纯化的过氧化物酶体一起孵育，导致其转化为一种极性更强的产物。相比之下，在相同条件下与线粒体部分和微粒体部分一起孵育未导致任何可检测到的转化。通过高效液相色谱法对从制备性孵育中获得的极性物质进行纯化，并通过放射性气相色谱法和气相色谱-质谱法对其进行表征。结果表明，该极性化合物的结构为5,7,11-三羟基-四降前列腺-9-烯酸（四降前列腺素F1α）。因此，前列腺素F2α的碳链缩短了四个碳原子。
