Sago T, Nakayama R, Okumura T, Saito K
Biochim Biophys Acta. 1986 Dec 5;879(3):330-8.
3H-Labeled prostaglandins D2 and F2 alpha rapidly degraded to more-polar metabolites in primary cultured rat hepatocytes. The metabolites of prostaglandins D2 and F2 alpha accumulated in the culture medium. The metabolites extracted by ethyl acetate at pH 3 were purified by silicic acid column and thin-layer chromatography of silica gel, and were analysed by gas chromatography-mass spectrometry. The major metabolites from prostaglandin D2 were identified as dinor-prostaglandin D1 (7 alpha,13-dihydroxy-9-ketodinorprost-11-enoic acid) and tetranor-prostaglandin D1 (5 alpha,11- dihydroxy-7-ketotetranorprost-9-enoic acid). Those from prostaglandin F2 alpha were identified as dinor-prostaglandin F1 alpha (7 alpha,9 alpha,13-trihydroxydinorprost-11-enoic acid), tetranor-prostaglandin F1 alpha (5 alpha,7 alpha,11-trihydroxytetranorprost-9-enoic acid) and 9 alpha,11 alpha,15-trihydroxyprost-13-ene-1,20-dioic acid. These data indicate that prostaglandins D2 and F2 alpha mainly degraded by beta-oxidation, which is the same process as reported earlier for prostaglandins E1 and E2, and that prostaglandin F2 alpha was also subjected to omega-oxidation.
3H标记的前列腺素D2和F2α在原代培养的大鼠肝细胞中迅速降解为极性更强的代谢产物。前列腺素D2和F2α的代谢产物在培养基中积累。用pH 3的乙酸乙酯萃取的代谢产物通过硅酸柱和硅胶薄层层析进行纯化，并通过气相色谱-质谱联用进行分析。前列腺素D2的主要代谢产物被鉴定为二降前列腺素D1（7α,13-二羟基-9-氧代二降前列腺-11-烯酸）和四降前列腺素D1（5α,11-二羟基-7-氧代四降前列腺-9-烯酸）。前列腺素F2α的主要代谢产物被鉴定为二降前列腺素F1α（7α,9α,13-三羟基二降前列腺-11-烯酸）、四降前列腺素F1α（5α,7α,11-三羟基四降前列腺-9-烯酸）和9α,11α,15-三羟基前列腺-13-烯-1,20-二酸。这些数据表明，前列腺素D2和F2α主要通过β-氧化降解，这与先前报道的前列腺素E1和E2的降解过程相同，并且前列腺素F2α也经历ω-氧化。
