Ji Yu-Bin, Dong Fang, Lang Lang, Zhang Ling-Wen, Miao Jing, Liu Zhen-Feng, Jin Li-Na, Hao Ying
Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China.
Int J Mol Sci. 2012 Dec 17;13(12):17275-89. doi: 10.3390/ijms131217275.
In this study, an experiment was designed to optimize the synthesis of seleno-Capparis spionosa L. polysaccharide (Se-CSPS) by response surface methodology. Three independent variables (reaction time, reaction temperature and ratio of Na(2)SeO(3) to CSPS) were tested. Furthermore, the thermal stability, particle size, shape and cytotoxic activity of Se-CSPS in vitro were investigated. The optimum reaction conditions were obtained shown as follows: reaction time 7.5 h, reaction temperature 71 °C, and ratio of Na(2)SeO(3) to CSPS 0.9 g/g. Under these conditions, the Se content in Se-CSPS reached 5.547 mg/g, which was close to the predicted value (5.518 mg/g) by the model. The thermal stability, particle size and shape of Se-CSPS were significantly different from those of CSPS. Additionally, a MTT assay indicated that the Se-CSPS could inhibit the proliferation of human gastric cancer SGC-7901 cells in a dose-dependent manner.
本研究设计了一个实验，采用响应面法优化硒化刺山柑多糖（Se-CSPS）的合成。测试了三个自变量（反应时间、反应温度和亚硒酸钠与刺山柑多糖的比例）。此外，还研究了Se-CSPS的热稳定性、粒径、形状及体外细胞毒性活性。获得的最佳反应条件如下：反应时间7.5小时、反应温度71℃、亚硒酸钠与刺山柑多糖的比例为0.9 g/g。在此条件下，Se-CSPS中的硒含量达到5.547 mg/g，接近模型预测值（5.518 mg/g）。Se-CSPS的热稳定性、粒径和形状与刺山柑多糖有显著差异。此外，MTT检测表明Se-CSPS能够以剂量依赖性方式抑制人胃癌SGC-7901细胞的增殖。
