Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou, PR China.
Int J Biol Macromol. 2012 Mar 1;50(2):348-52. doi: 10.1016/j.ijbiomac.2011.12.019. Epub 2011 Dec 24.
In this study, the water-soluble selenium-enriched exopolysaccharides (Se-ECZ-EPS) were isolated from submerged culture broth of Enterobacter cloacae Z0206 through fermentation, ethanol precipitation and deproteinization. The protective effects of Se-ECZ-EPS on alloxan-induced diabetic mice were investigated. Diabetes was induced in ICR (Institute of Cancer Research) mice by administration of single doses of alloxan intraperitoneally (190 mg/kg body weight). Se-ECZ-EPS at a dose of 200 mg/kg body weight were administered per os (p.o.) as single dose per day to diabetes-induced mice for a period of 42 days. The decrease in body weight, serum insulin level, and the increase in blood glucose level, glycosylated serum protein (GSP), total cholesterol (TC) and triglycerides (TG) in liver were observed in diabetic mice. On the other hand, oral administration of Se-ECZ-EPS resulted in a significant reduction in fasting blood glucose levels, GSP, TC and TG contents in liver coupled with improvement of body weight and serum insulin level in comparison with diabetic control group. These results suggest that Se-ECZ-EPS possess significant protective and anti-diabetic effects in alloxan-induced diabetic mice.
在这项研究中，通过发酵、乙醇沉淀和脱蛋白从阴沟肠杆菌 Z0206 的发酵液中分离出水溶性富硒胞外多糖（Se-ECZ-EPS）。研究了 Se-ECZ-EPS 对丙烯醛诱导的糖尿病小鼠的保护作用。通过腹腔内单次给予丙烯醛（190mg/kg 体重）诱导 ICR（癌症研究所）小鼠糖尿病。以 200mg/kg 体重的剂量每天口服给予糖尿病诱导的小鼠单剂量 Se-ECZ-EPS，持续 42 天。糖尿病小鼠的体重下降、血清胰岛素水平升高以及血糖、糖化血清蛋白（GSP）、总胆固醇（TC）和肝甘油三酯（TG）升高。另一方面，与糖尿病对照组相比，口服 Se-ECZ-EPS 可显著降低空腹血糖水平、GSP、TC 和 TG 含量，并改善体重和血清胰岛素水平。这些结果表明，Se-ECZ-EPS 对丙烯醛诱导的糖尿病小鼠具有显著的保护和抗糖尿病作用。
