Rajiv Gandhi University of Health Sciences, 4 T Block, Jayanagar, Bangalore, Karnataka, India.
Indian J Pharmacol. 2012 Nov-Dec;44(6):732-6. doi: 10.4103/0253-7613.103273.
The anticancer activity of shatavarins (containing shatavarin IV) isolated from the roots of Asparagus racemosus (Wild) was evaluated using in vitro and in vivo experimental models.
The shatavarin IV was isolated from ethyl acetate insoluble fraction (AR-2B) of chloroform:methanol (2:1) (AR-2) extract of A. racemosus roots. The cytotoxicity (in vitro) of shatavarin IV and other shatavarins rich fraction was carried out using of MTT assay using MCF-7 (human breast cancer), HT-29 (human colon adenocarcinoma), and A-498 (human kidney carcinoma) cell lines. The in vivo anticancer activity of shatavarins (containing shatavarin IV) was evaluated against Ehrlich ascites carcinoma (EAC) tumor bearing mice.
The isolated shatavarin IV (84.69 %) along with shatavarins rich fraction, coded AR-2B containing 5.05% shatavarin IV showed potent cytotoxicity. Oral administration of AR-2B to tumor bearing mice at doses of 250 and 500 mg/kg body weight for 10 days, showed significant reduction in percent increase in body weight, tumor volume, packed cell volume, viable tumor cell count, and increased non-viable cell count when compared to the untreated mice of the EAC control group. The restoration of hematological parameters towards normalcy was also observed.
The result suggests that the shatavarins (containing shatavarin IV) rich fraction (AR-2B) exhibits significant anticancer activity in both in vitro and in vivo experimental models.
评估从天门冬科植物天门冬（野天门冬）根部分离得到的沙塔瓦林（含有沙塔瓦林 IV）的抗癌活性，使用体外和体内实验模型进行评估。
从氯仿:甲醇（2:1）（AR-2）提取物的乙酸乙酯不溶部分（AR-2B）中分离出沙塔瓦林 IV。使用 MTT 测定法，通过 MCF-7（人乳腺癌）、HT-29（人结肠腺癌）和 A-498（人肾癌细胞系）进行沙塔瓦林 IV 和其他富含沙塔瓦林的部分的细胞毒性（体外）测定。评价沙塔瓦林（含有沙塔瓦林 IV）对艾氏腹水癌（EAC）荷瘤小鼠的体内抗癌活性。
分离得到的沙塔瓦林 IV（84.69%）和富含沙塔瓦林的部分 AR-2B（含有 5.05%的沙塔瓦林 IV）表现出很强的细胞毒性。AR-2B 以 250 和 500mg/kg 体重的剂量口服给予荷瘤小鼠 10 天，与未经处理的 EAC 对照组相比，体重增加百分比、肿瘤体积、红细胞压积、存活肿瘤细胞计数显著减少，非存活细胞计数增加。还观察到血液学参数向正常化的恢复。
结果表明，富含沙塔瓦林（含有沙塔瓦林 IV）的部分（AR-2B）在体外和体内实验模型中均表现出显著的抗癌活性。
