Vijayan P, Vinod Kumar S, Dhanaraj S A, Mukherjee P K, Suresh B
J.S.S. College of Pharmacy, Rocklands, Ootacamund--643 001, Tamilnadu, India.
Phytother Res. 2003 Sep;17(8):952-6. doi: 10.1002/ptr.1271.
The methanol extracts of the aerial parts of Hypericum mysorense and Hypericum patulum were tested for in vitro cytotoxicity on HEp-2, RD and Vero cell lines and antitumour activity using DLA and HEp-2 cell lines. The cell viability and morphological changes were assessed. Of these extracts, Hypericum patulum (stem) extract showed strong cytotoxicity against all the cell lines used. The CTC50 of the Hypericum patulum (stem) extract was 1.71 microg/mL for HEp-2, 1.53 microg/mL for RD and 2.23 microg/mL for Vero cell lines. The Hypericum patulum (leaves) and Hypericum mysorense (aerial parts) extracts showed moderate cytotoxicity and Hypericum patulum (aerial parts) extract did not show any cytotoxicity up to 1,000 microg/mL concentration. In the clonogenic assay, no colony formation was observed at a concentration of 300 micro g/mL and above for Hypericum mysorense (aerial parts), 400 microg/mL and above for Hypericum patulum (leaves) and 500 microg/mL and above for Hypericum patulum (stem) extracts. In the short term antitumour studies using DLA cells, 50% viability was observed in the concentration range 100-200 microg/mL for Hypericum patulum (leaves and stem) and 200-400 microg/mL for Hypericum mysorense (aerial) extract. In the long term antitumour activity using the HEp-2 cell line, no colony formation was observed over a concentration of 1.6 microg/mL for the Hypericum patulum (stem) extract.
对金丝桃和赶山鞭地上部分的甲醇提取物进行了体外细胞毒性测试，测试对象为HEp-2、RD和Vero细胞系，并使用DLA和HEp-2细胞系进行了抗肿瘤活性测试。评估了细胞活力和形态变化。在这些提取物中，赶山鞭（茎）提取物对所有使用的细胞系均表现出强烈的细胞毒性。赶山鞭（茎）提取物对HEp-2细胞系的半数细胞毒性浓度（CTC50）为1.71微克/毫升，对RD细胞系为1.53微克/毫升，对Vero细胞系为2.23微克/毫升。赶山鞭（叶）和金丝桃地上部分提取物表现出中等细胞毒性，赶山鞭地上部分提取物在浓度高达1000微克/毫升时未表现出任何细胞毒性。在克隆形成试验中，金丝桃地上部分提取物在浓度为300微克/毫升及以上时未观察到集落形成，赶山鞭（叶）提取物在400微克/毫升及以上时未观察到集落形成，赶山鞭（茎）提取物在500微克/毫升及以上时未观察到集落形成。在使用DLA细胞的短期抗肿瘤研究中，赶山鞭（叶和茎）提取物在浓度范围为100-200微克/毫升时观察到50%的细胞活力，金丝桃地上部分提取物在浓度范围为200-400微克/毫升时观察到50%的细胞活力。在使用HEp-2细胞系的长期抗肿瘤活性研究中，赶山鞭（茎）提取物在浓度超过1.6微克/毫升时未观察到集落形成。
