Bertani-Dziedzic L M, Krstulovic A M, Ciriello S, Gitlow S E
J Chromatogr. 1979 Nov 11;164(3):345-53. doi: 10.1016/s0378-4347(00)81235-9.
A rapid and simple reversed-phase high-performance liquid chromatographic procedure for the determination of vanillylmandelic acid (VMA) is described. This method was applied in the determination of the VMA content in urine from normal subjects and patients with neural crest lesions. Sample preparation is minimal and the analysis is short (20 min) and reproducible. The sensitivity of the UV detection is in the ng range. By this technique, fourteen adult control subjects were found to excrete a mean of 2.86 microgram VMA per mg creatinine, whereas twelve patients with pheochromocytoma excreted a mean of 15.7 microgram VMA per mg creatinine.
本文描述了一种快速简便的反相高效液相色谱法，用于测定香草扁桃酸（VMA）。该方法应用于正常受试者和神经嵴病变患者尿液中VMA含量的测定。样品制备简单，分析时间短（20分钟）且可重复。紫外检测的灵敏度在纳克范围内。通过该技术，发现14名成年对照受试者每毫克肌酐平均排泄2.86微克VMA，而12名嗜铬细胞瘤患者每毫克肌酐平均排泄15.7微克VMA。
