Laboratoire des Substances Bioactives Centre de Biotechnologies à la Technoplole de Borj-Cédria (CBBC), Hammam-Lif, Tunisia.
J Agric Food Chem. 2011 Jan 12;59(1):328-34. doi: 10.1021/jf1037618. Epub 2010 Dec 8.
This study is designed to examine the effect of water deficit on growth, fatty acid and essential oil composition, and antioxidant activities of Cuminum cyminum aerial part extracts. Plants were treated with different levels of water deficit: control (C), moderate water deficit (MWD), and severe water deficit (SWD). Plant growth (height, fresh and dry matter weights) as well as yield components were significantly increased under moderate water deficit and conversely reduced at severe level. Total fatty acid content decreased significantly with severity of constraint. Drought reduced considerably the proportions of major fatty acids and the unsaturated to saturated fatty acid ratio. The essential oil yield was 0.14% (based on the dry weight); it increased by 2.21-fold at MWD but decreased by 42.8% under SWD in comparison to the control. Drought results in the modification of the essential oil chemotype from 1-phenyl-1-butanol to 1-phenyl-1,2-ethanediol. Antioxidant activities of the acetone extracts were determined by two complementary test systems, namely, DPPH and β-carotene/linoleic acid. The highest activity was exhibited by moderately stressed plants and was reduced significantly under SWD. In control plants, the total phenolic amount was 10.23 mg GAE/g DW, which increased by 1.5-fold under MWD and decreased by 42% under SWD.
本研究旨在探讨水分胁迫对孜然地上部分提取物的生长、脂肪酸和精油组成以及抗氧化活性的影响。将植物分别施以不同程度的水分胁迫：对照（C）、中度水分胁迫（MWD）和重度水分胁迫（SWD）。中度水分胁迫下，植物的生长（高度、鲜重和干重）和产量均显著增加，而重度胁迫下则相反。总脂肪酸含量随胁迫程度的增加而显著降低。干旱显著降低了主要脂肪酸的比例和不饱和与饱和脂肪酸的比值。精油得率为 0.14%（以干重计）；与对照相比，MWD 下增加了 2.21 倍，而 SWD 下则减少了 42.8%。干旱导致精油化学型从 1-苯-1-丁醇变为 1-苯-1,2-乙二醇。采用两种互补的测试系统，即 DPPH 和β-胡萝卜素/亚油酸，测定了丙酮提取物的抗氧化活性。中度胁迫下植物的抗氧化活性最高，重度胁迫下显著降低。在对照植物中，总酚含量为 10.23 mg GAE/g DW，MWD 下增加了 1.5 倍，SWD 下则减少了 42%。
