van Buul P P, Goudzwaard J H
Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
Mutat Res. 1990 Apr;243(4):259-66. doi: 10.1016/0165-7992(90)90141-6.
The induction of reciprocal translocations in mouse spermatogonial stem cells, visualised in dividing primary spermatocytes, was studied after combined treatments with hydroxyurea (250 and 500 mg/kg) and X-rays (6, 8 and 9 Gy). The time intervals between the 2 treatments were 16 h (leading to extremely high cell killing) and 48 h (giving rise to less killing than irradiation alone). Comparison of the observed frequencies of translocations with reported data on stem cell killing (de Ruiter-Bootsma and Davids, 1981) show that the ratio between the probabilities that a radiation-induced basic lesion kills a cell or produces a translocation, theoretically calculated by Leenhouts and Chadwick (1981) to be about 10, can indeed be confirmed experimentally.
在用羟基脲（250和500mg/kg）和X射线（6、8和9Gy）联合处理后，研究了在分裂的初级精母细胞中可视化的小鼠精原干细胞中相互易位的诱导情况。两种处理之间的时间间隔分别为16小时（导致极高的细胞杀伤率）和48小时（产生的杀伤率低于单独照射）。将观察到的易位频率与已报道的干细胞杀伤数据（de Ruiter - Bootsma和Davids，1981年）进行比较，结果表明，Leenhouts和Chadwick（1981年）理论计算得出的辐射诱导的基本损伤杀死细胞或产生易位的概率之比约为10，确实可以通过实验得到证实。
