van Buul P P, Schöppink K, de Rooij D G, Seelen M C
MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratory, The Netherlands.
Radiat Res. 1994 Feb;137(2):171-6.
Dose-response relationships for X-ray-induced reciprocal translocations in spermatogonial stem cells of mutant and wild-type mice were established by spermatocyte analysis many cell generations after irradiation. The mutants studied were Wv/+, the viable allele of dominant spotting in the heterozygous state, and Slcon/Slcon, the homozygous contrasted allele of steel. The results show that the recovered yield of translocations was lowered in both mutants with steel being most extreme. Remarkably, however, no indications for enhanced cell killing were obtained in the mutants, and consequently the peak yields of translocations occurred at about the same dose level (6 Gy) as in normal mice. Histological analysis suggested that the postirradiation recovery of the germinal epithelium was retarded in the mutants with the effect in the steel mice again being most extreme. These differences in differentiation-multiplication patterns of regenerating spermatogonia after irradiation are probably responsible for the reduced recovery of translocations from the mutant mice.
通过照射后多代细胞的精母细胞分析，建立了突变型和野生型小鼠精原干细胞中X射线诱导的相互易位的剂量反应关系。所研究的突变体是杂合状态下显性斑点的存活等位基因Wv/+，以及钢的纯合对照等位基因Slcon/Slcon。结果表明，两种突变体中易位的回收产量均降低，钢突变体最为明显。然而，值得注意的是，在突变体中未发现细胞杀伤增强的迹象，因此易位的峰值产量出现在与正常小鼠大致相同的剂量水平（6 Gy）。组织学分析表明，突变体中生发上皮的照射后恢复受到阻碍，钢小鼠中的影响再次最为明显。照射后再生精原细胞分化增殖模式的这些差异可能是突变小鼠易位回收减少的原因。
