Van Buul P P, De Rooij D G, Zandman I M, Grigorova M, Van Duyn-Goedhart A
MGC-Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratory, Leiden, The Netherlands.
Int J Radiat Biol. 1995 May;67(5):549-55. doi: 10.1080/09553009514550661.
To characterize further the radiosensitivity of severe combined immunodeficiency (scid) mice, the induction of micronuclei (MN) in polychromatic erythrocytes as well as cell killing and translocation induction in stem cell spermatogonia was studied. Scid mice turned out to be clearly hypersensitive for X-ray-induced cell killing of both bonemarrow cells and spermatogonial stem cells. The frequencies of recorded micronuclei in polychromatic erythrocytes were comparable with that reported for the normal mouse, whereas the recovery of translocations was extremely low in the scid mouse. The dose-response relationship for induced translocations was bell shaped with a maximum of about 0.5% around doses of 0.5-1.5 Gy X-rays.
为了进一步表征严重联合免疫缺陷（scid）小鼠的辐射敏感性，研究了多色红细胞中微核（MN）的诱导以及干细胞精原细胞中的细胞杀伤和易位诱导。结果表明，scid小鼠对X射线诱导的骨髓细胞和精原干细胞的细胞杀伤明显敏感。多色红细胞中记录的微核频率与正常小鼠报道的频率相当，而scid小鼠中易位的恢复率极低。诱导易位的剂量-反应关系呈钟形，在0.5-1.5 Gy X射线剂量左右，最大值约为0.5%。
