[1,3 - 丁二烯暴露工人尿液生物标志物的研究]

[Study on urine biomarkers in 1,3-butadiene exposed workers].

作者信息

Cheng Xue-mei, Jiao Yan-ni, Chen Jin-dong, Shan Bao-de, Xia Zhao-lin

机构信息

Fudan University, Public Health School, Shanghai 200032, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2012 Sep;30(9):661-6.

PMID:23257089

Abstract

OBJECTIVE

To discuss the urine biomarkers in 1,3-butadiene exposed workers, and to provide basement for establishing biological limit value.

METHODS

44 BD exposed workers as exposure group and 25 BD non-exposed people as control group including 12 workers in boiler workshop in the same factory and 13 people in one public institute, we collected their in-end-of shift urine, then detected urine BD-derived mercapturic metabolites [3,4-dihydroxybutyl mercapturic acid (DHBMA),1- and 2-monohydroxy-3-butenyl mercapturic acid (MHBMA)] concentrations using UPLC-MS/MS method. Meanwhile, we detected air BD concentration with GC-FID in the workplace, and compared their relationship.

RESULTS

lgDHBMA and lg (MHBMA + DHBMA) levels in exposed group (lgDHBMA: 2.51 ± 0.44) µg/L, lg [MHBMA + DHBMA: (2.68 ± 0.27) µg/L] were higher than which in control group (lgDHBMA: (2.20 ± 0.25) µg/L, lg(MHBMA + DHBMA: (2.49 ± 0.34) µg/L), and the differences were significant (P < 0.01). Urine DHBMA was obviously influenced by air BD concentrations (r = 0.539, P = 0.001). The equation of Multiple Regression Analysis was y = 2.417 + 0.520x (x represents air BD dose, and represents urinary DHBMA level). Adjusted R(2) of this model was 0.262. Urinary MHBMA was not affected by smoking, alcohol and years of works.

CONCLUSION

Urine metabolite DHBMA in BD-exposed workers might be major biological exposure indice.

摘要

目的

探讨1,3 - 丁二烯暴露工人的尿液生物标志物，为制定生物接触限值提供依据。

方法

选取44名丁二烯暴露工人作为暴露组，25名未接触丁二烯人员作为对照组（包括同一工厂锅炉车间12名工人和某事业单位13人），收集其下班时尿液，采用超高效液相色谱 - 串联质谱法检测尿液中丁二烯衍生的巯基尿酸代谢物[3,4 - 二羟基丁基巯基尿酸（DHBMA）、1 - 和2 - 单羟基 - 3 - 丁烯基巯基尿酸（MHBMA）]浓度。同时，用气相色谱 - 氢火焰离子化检测器检测工作场所空气中丁二烯浓度，并比较两者关系。

结果

暴露组lgDHBMA和lg（MHBMA + DHBMA）水平[lgDHBMA：（2.51±0.44）μg/L，lg（MHBMA + DHBMA）：（2.68±0.27）μg/L]高于对照组[lgDHBMA：（2.20±0.25）μg/L，lg（MHBMA + DHBMA）：（2.49±0.34）μg/L]，差异有统计学意义（P < 0.01）。尿DHBMA明显受空气中丁二烯浓度影响（r = 0.539，P = 0.001）。多元回归分析方程为y = 2.417 + 0.520x（x代表空气中丁二烯剂量，y代表尿DHBMA水平）。该模型的调整R²为0.262。尿MHBMA不受吸烟、饮酒和工作年限影响。