Division of Environmental Engineering, Hokkaido University, Japan.
Microbes Environ. 2013;28(1):96-104. doi: 10.1264/jsme2.me12141. Epub 2012 Dec 19.
The removal of biodegradable organic matter is one of the most important objectives in biological wastewater treatments. Polyhydroxyalkanoate (PHA)-accumulating organisms (PHAAOs) significantly contribute to the removal of biodegradable organic matter; however, their microbial community composition is mostly unknown. In the present study, the microbial community composition of PHAAOs was investigated at 8 full-scale wastewater treatment plants (WWTPs), operated in fully aerobic mode, by fluorescence in situ hybridization (FISH) analysis and post-FISH Nile blue A (NBA) staining techniques. Our results demonstrated that 1) PHAAOs were in the range of 11-18% in the total number of cells, and 2) the microbial community composition of PHAAOs was similar at the bacterial domain/phylum/class/order level among the 8 full-scale WWTPs, and dominant PHAAOs were members of the class Alphaproteobacteria and Betaproteobacteria. The microbial community composition of α- and β-proteobacterial PHAAOs was examined by 16S rRNA gene clone library analysis and further by applying a set of newly designed oligonucleotide probes targeting 16S rRNA gene sequences of α- or β-proteobacterial PHAAOs. The results demonstrated that the microbial community composition of PHAAOs differed in the class Alphaproteobacteria and Betaproteobacteria, which possibly resulted in a different PHA accumulation capacity among the WWTPs (8.5-38.2 mg-C g-VSS(-1) h(-1)). The present study extended the knowledge of the microbial diversity of PHAAOs in full-scale WWTPs operated in fully aerobic mode.
去除可生物降解的有机物是生物废水处理的最重要目标之一。聚羟基烷酸酯（PHA）积累菌（PHAAOs）对可生物降解有机物的去除有重要贡献，但它们的微生物群落组成大多未知。在本研究中，通过荧光原位杂交（FISH）分析和 FISH 后尼罗蓝 A（NBA）染色技术，对 8 个全规模污水处理厂（WWTPs）中 PHAAOs 的微生物群落组成进行了研究。研究结果表明：1）PHAAOs 在总细胞数中的比例为 11-18%；2）在 8 个全规模 WWTPs 中，PHAAOs 的细菌域/门/纲/目水平的微生物群落组成相似，优势 PHAAOs 为α变形菌纲和β变形菌纲的成员。通过 16S rRNA 基因克隆文库分析和应用一组新设计的针对α或β变形菌 PHAAOs 16S rRNA 基因序列的寡核苷酸探针，进一步研究了α-和β-变形菌 PHAAOs 的微生物群落组成。结果表明，PHAAOs 的微生物群落组成在α变形菌纲和β变形菌纲中存在差异，这可能导致 WWTPs 之间的 PHA 积累能力不同（8.5-38.2 mg-C g-VSS(-1) h(-1)）。本研究扩展了全规模 WWTPs 中 PHAAOs 的微生物多样性知识，这些 WWTPs 采用完全好氧模式运行。
