Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.
Nat Commun. 2021 Mar 31;12(1):2009. doi: 10.1038/s41467-021-22203-2.
Microorganisms play crucial roles in water recycling, pollution removal and resource recovery in the wastewater industry. The structure of these microbial communities is increasingly understood based on 16S rRNA amplicon sequencing data. However, such data cannot be linked to functional potential in the absence of high-quality metagenome-assembled genomes (MAGs) for nearly all species. Here, we use long-read and short-read sequencing to recover 1083 high-quality MAGs, including 57 closed circular genomes, from 23 Danish full-scale wastewater treatment plants. The MAGs account for ~30% of the community based on relative abundance, and meet the stringent MIMAG high-quality draft requirements including full-length rRNA genes. We use the information provided by these MAGs in combination with >13 years of 16S rRNA amplicon sequencing data, as well as Raman microspectroscopy and fluorescence in situ hybridisation, to uncover abundant undescribed lineages belonging to important functional groups.
微生物在废水处理行业的水循环、污染去除和资源回收中发挥着关键作用。基于 16S rRNA 扩增子测序数据，人们越来越了解这些微生物群落的结构。然而，如果没有几乎所有物种的高质量宏基因组组装基因组（MAG），这些数据就无法与功能潜力联系起来。在这里，我们使用长读长和短读长测序从 23 个丹麦全规模废水处理厂中回收了 1083 个高质量 MAG，其中包括 57 个闭合环状基因组。根据相对丰度，MAG 占群落的~30%，并满足严格的 MIMAG 高质量草案要求，包括全长 rRNA 基因。我们利用这些 MAG 提供的信息，结合超过 13 年的 16S rRNA 扩增子测序数据，以及拉曼微光谱和荧光原位杂交，揭示了丰富的未描述的属于重要功能群的谱系。
