State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangzhou, China.
Invest Ophthalmol Vis Sci. 2013 Jan 28;54(1):804-12. doi: 10.1167/iovs.12-9695.
We investigated whether the foldable capsular vitreous body (FCVB) could release levofloxacin sustainably in vitro and inhibit endophthalmitis in rabbit models.
Approximately 1.0 mL levofloxacin (625 μg/mL) was injected into the capsule of nine FCVBS. The levofloxacin release value was determined in the modified franz diffusion cells over time. In the in vivo study, all right eyes of 45 rabbits were infected with Staphylococcus epidermidis AND were divided randomly into three groups at 24 hours after infection: FCVB plus levofloxacin (n = 15), silicone oil plus subconjunctival levofloxacin (n = 15), and an untreated group (n = 15) during a 30-day observation time. Levofloxacin concentrations in the aqueous humor were detected, and therapeutic efficacy was evaluated with clinical evaluation, bacterial counts, cytokine profiles, and histopathology.
The FCVB released levofloxacin ranging from 9 to 13.5 ng/mL in vitro and from 42 to 1.6 ng/mL in the aqueous humor during 30 days. In the FCVB and silicone-treated groups, clinical inflammation almost was abolished; no bacteria were detected in the aqueous humor; TNF-α, IL-1β, and IFN-γ expression decreased; and relatively normal corneal and retinal architecture were kept after the 30-day treatment.
The FCVB could provide us with dual functions, combining a levofloxacin drug delivery system and a vitreous substitute, to treat endophthalmitis in rabbit eyes.
我们研究了可折叠囊玻璃体（FCVB）是否能在体外持续释放左氧氟沙星，并抑制兔眼内炎模型中的内毒素。
将约 1.0 毫升左氧氟沙星（625μg/ml）注入 9 个 FCVBS 囊内。在改良的 Franz 扩散池内，随时间确定左氧氟沙星的释放值。在体内研究中，所有 45 只兔的右眼均感染表皮葡萄球菌，并在感染后 24 小时随机分为三组：FCVB 加左氧氟沙星（n=15）、硅油加球结膜下左氧氟沙星（n=15）和未治疗组（n=15），观察 30 天。检测房水中的左氧氟沙星浓度，并通过临床评估、细菌计数、细胞因子谱和组织病理学评估治疗效果。
FCVB 在体外释放左氧氟沙星范围为 9 至 13.5ng/ml，在 30 天内房水中释放 42 至 1.6ng/ml。在 FCVB 和硅油治疗组中，临床炎症几乎完全消退；房水中未检出细菌；TNF-α、IL-1β 和 IFN-γ 表达降低；经过 30 天治疗后，角膜和视网膜结构相对正常。
FCVB 可提供双重功能，结合左氧氟沙星药物输送系统和玻璃体替代物，治疗兔眼内炎。
