Department of Ophthalmology, Medical University of Vienna, Austria.
Invest Ophthalmol Vis Sci. 2013 Jan 28;54(1):739-45. doi: 10.1167/iovs.11-7877.
To investigate the reproducibility of automated lesion size detection in patients with geographic atrophy (GA) using polarization-sensitive spectral-domain optical coherence tomography (PS-OCT) and to compare findings with scanning laser ophthalmoscopy (SLO), fundus autofluorescence (FAF), and intensity-based spectral-domain OCT (SD-OCT).
Twenty-nine eyes of 22 patients with GA were examined by PS-OCT, selectively identifying the retinal pigment epithelium (RPE). A novel segmentation algorithm was applied, automatically detecting and quantifying areas of RPE atrophy. The reproducibility of the algorithm was assessed, and lesion sizes were correlated with manually delineated SLO, FAF, and intensity-based SD-OCT images to validate the clinical applicability of PS-OCT in GA evaluation.
Mean GA lesion size of all patients was 5.28 mm(2) (SD: 4.92) in PS-OCT. Mean variability of individual repeatability measurements was 0.83 mm(2) (minimum: 0.05; maximum: 3.65). Mean coefficient of variation was 0.07 (min: 0.01; max: 0.19). Mean GA area in SLO (Spectralis OCT) was 5.15 mm(2) (SD: 4.72) and 2.5% smaller than in PS-OCT (P = 0.9, Pearson correlation coefficient = 0.98, P < 0.01). Mean GA area in intensity-based SD-OCT pseudo-SLO images (Cirrus OCT) was 5.14 mm(2) (SD: 4.67) and 2.7% smaller than in PS-OCT (P = 0.9, Pearson correlation coefficient = 0.98, P < 0.01). Mean GA area of all eyes measured 5.41 mm(2) (SD: 4.75) in FAF, deviating by 2.4% from PS-OCT results (P = 0.89, Pearson correlation coefficient = 0.99, P < 0.01).
PS-OCT demonstrated high reproducibility of GA lesion size determination. Results correlated well with SLO, FAF, and intensity-based SD-OCT fundus imaging. PS-OCT may therefore be a valuable and specific imaging modality for automated GA lesion size determination in scientific studies and clinical practice.
利用偏振敏感光谱域光学相干断层扫描（PS-OCT）研究地理萎缩（GA）患者的自动病变大小检测的重现性，并与扫描激光检眼镜（SLO）、眼底自发荧光（FAF）和基于强度的光谱域 OCT（SD-OCT）进行比较。
对 22 例 29 只 GA 患者的眼睛进行 PS-OCT 检查，选择性识别视网膜色素上皮（RPE）。应用一种新的分割算法，自动检测和量化 RPE 萎缩区域。评估算法的重现性，并将病变大小与手动描绘的 SLO、FAF 和基于强度的 SD-OCT 图像相关联，以验证 PS-OCT 在 GA 评估中的临床适用性。
所有患者的平均 GA 病变大小为 5.28mm²（标准差：4.92），PS-OCT 中。个体重复性测量的平均可变性为 0.83mm²（最小值：0.05；最大值：3.65）。平均变异系数为 0.07（最小值：0.01；最大值：0.19）。SLO（Spectralis OCT）中的平均 GA 面积为 5.15mm²（标准差：4.72），比 PS-OCT 小 2.5%（P=0.9，Pearson 相关系数=0.98，P<0.01）。基于强度的 SD-OCT 伪 SLO 图像（Cirrus OCT）中的平均 GA 面积为 5.14mm²（标准差：4.67），比 PS-OCT 小 2.7%（P=0.9，Pearson 相关系数=0.98，P<0.01）。FAF 中所有眼睛的平均 GA 面积为 5.41mm²（标准差：4.75），与 PS-OCT 结果相差 2.4%（P=0.89，Pearson 相关系数=0.99，P<0.01）。
PS-OCT 显示 GA 病变大小测定具有高度的重现性。结果与 SLO、FAF 和基于强度的 SD-OCT 眼底成像相关性良好。因此，PS-OCT 可能是一种有价值的、专门的成像方式，可用于科学研究和临床实践中自动 GA 病变大小的测定。
