Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Yuhangtang Road 388, Hangzhou 310058, China.
J Hered. 2013 Mar;104(2):287-94. doi: 10.1093/jhered/ess103. Epub 2012 Dec 20.
In an earlier greenhouse screening, we identified a local indica cultivar HT54 tolerant to high temperature at both seedling and grain-filling stages. In this study, we develop an optimized procedure for fine assessment of this heat tolerance. The results indicated that HT54 seedlings could tolerate high temperature up to 48 °C for 79h. The genetic analysis of F(1) and F(2) offspring derived from the cross between HT54 and HT13, a heat-sensitive breeding line, reveals that the heat tolerance of HT54 was controlled by a dominant major locus, which has been designated as OsHTAS (Oryza sativa heat tolerance at seedling stage). This locus was mapped on rice chromosome 9 within an interval of 420kb between markers of InDel5 and RM7364. The determined candidate ZFP gene has been confirmed to be cosegregated with a single nucleotide polymorphism (SNP) developed PCR-restriction fragment length polymorphism (RFLP) marker RBsp1407 in its promoter region. Another heat tolerance-associated SNP was identified in the first intron of its 5'-untranslated region. The existence of these SNPs thereby indicated that the OsHTAS locus contains at least two alleles. We named the one from HT54 as OsHTAS ( a ) and the one from HT13 as OsHTAS ( b ). Further dynamic expression analysis demonstrated that OsHTAS ( a ) was actively responsive to 45 °C high temperature stress compared with the OsHTAS ( b ) allele.
在早期的温室筛选中，我们鉴定出一个地方 indica 品种 HT54 在幼苗期和灌浆期都能耐受高温。在这项研究中，我们开发了一种优化的程序来精细评估这种耐热性。结果表明，HT54 幼苗可以耐受高达 48°C 的高温 79 小时。HT54 与 HT13（一种热敏品系）杂交产生的 F(1)和 F(2)后代的遗传分析表明，HT54 的耐热性由一个显性主基因座控制，该基因座被命名为 OsHTAS（水稻幼苗期耐热性）。该基因座位于水稻 9 号染色体上，位于 InDel5 和 RM7364 标记之间的 420kb 区间内。确定的候选 ZFP 基因已被证实与启动子区域内的单核苷酸多态性（SNP）RBsp1407 开发的 PCR-限制性片段长度多态性（RFLP）标记共分离。在其 5'非翻译区的第一个内含子中还发现了另一个与耐热性相关的 SNP。这些 SNP 的存在表明 OsHTAS 基因座至少包含两个等位基因。我们将 HT54 中的一个命名为 OsHTAS(a)，将 HT13 中的一个命名为 OsHTAS(b)。进一步的动态表达分析表明，与 OsHTAS(b)等位基因相比，OsHTAS(a)在 45°C 高温胁迫下的表达更为活跃。
