Siedlecka-Kroplewska Kamila, Jozwik Agnieszka, Kaszubowska Lucyna, Kowalczyk Anna, Boguslawski Wojciech
Department of Histology, Medical University of Gdansk, Gdansk, Poland.
Folia Histochem Cytobiol. 2012;50(4):574-80. doi: 10.5603/20257.
Pterostilbene, a polyphenolic compound present in grapes and other fruits, has been demonstrated to inhibit growth and induce apoptosis and autophagy in some cancer cell types. We found that pterostilbene at the IC(90) concentration of 44 µM inhibited proliferation and induced apoptosis in MOLT4 human leukemia cells. Treatment with pterostilbene resulted in a transient accumulation of cells in the G(0)/G(1)-cell cycle phase followed by the S-phase arrest. Pterostilbene-induced apoptotic death of MOLT4 cells was mediated by caspase-3 activation and was accompanied by the disruption of mitochondrial membrane potential, phosphatidylserine externalization and internucleosomal DNA fragmentation. Our results suggest that pterostilbene could serve as a potential additional chemotherapeutic agent for the treatment of leukemia.
紫檀芪是一种存在于葡萄和其他水果中的多酚类化合物，已被证明能抑制某些癌细胞类型的生长并诱导其凋亡和自噬。我们发现，紫檀芪在44µM的IC(90)浓度下可抑制MOLT4人白血病细胞的增殖并诱导其凋亡。用紫檀芪处理导致细胞在G(0)/G(1)细胞周期阶段短暂积累，随后出现S期阻滞。紫檀芪诱导的MOLT4细胞凋亡性死亡是由半胱天冬酶-3激活介导的，并伴有线粒体膜电位的破坏、磷脂酰丝氨酸外翻和核小体间DNA片段化。我们的结果表明，紫檀芪可作为治疗白血病的一种潜在的辅助化疗药物。
