Lungu Cristina, Mihai Cosmin-Teodor, Vochita Gabriela, Gherghel Daniela, Mangalagiu Ionel I, Gafton Mihaela, Miron Sorin-Dan, Iurciuc Tincu Camelia-Elena, Nahar Lutfun, Sarker Satyajit D, Miron Anca
Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy, 16, Universitatii Street, 700115 Iasi, Romania.
Institute of Biological Research Iasi, Branch of NIRDBS-National Institute of Research and Development for Biological Sciences, 47, Lascar Catargi Street, 700107 Iasi, Romania.
Plants (Basel). 2025 May 14;14(10):1459. doi: 10.3390/plants14101459.
Stilbenes are plant secondary metabolites with remarkable antidiabetic, anti-inflammatory, antimicrobial, antioxidant, antitumor, and neuroprotective properties. As these compounds are valuable constituents in healthcare products and promising drug candidates, exploring new sources of stilbenes is essential for therapeutic advancement. The present study reports the isolation of two stilbene glycosides, resveratroloside and pinostilbenoside, from L. bark. Their antioxidant activity and cytotoxic effects against HeLa cells were evaluated in comparison to the raw bark extract. The structures of resveratroloside and pinostilbenoside were confirmed by nuclear magnetic resonance (NMR) and mass spectrometry (MS) data analyses. Antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and reducing power assays. Cell viability, apoptosis, cell proliferation, and cell cycle assays were used to evaluate the cytotoxic potential against HeLa cells. Resveratroloside and pinostilbenoside exhibited lower activity as free radical scavengers and reducing agents. However, they showed greater efficacy in reducing viability and suppressing proliferation in human cervical carcinoma HeLa cells. Given the promising findings of our study, the therapeutic potential of resveratroloside and pinostilbenoside should be further investigated.
芪类化合物是植物次生代谢产物，具有显著的抗糖尿病、抗炎、抗菌、抗氧化、抗肿瘤和神经保护特性。由于这些化合物是保健品中的重要成分和有前景的候选药物，探索芪类化合物的新来源对于治疗进展至关重要。本研究报道了从L.树皮中分离出两种芪糖苷，白藜芦醇苷和松 stilbenoside。与生树皮提取物相比，评估了它们对HeLa细胞 的抗氧化活性和细胞毒性作用。通过核磁共振（NMR）和质谱（MS）数据分析证实了白藜芦醇苷和松 stilbenoside 的结构。通过2,2-二苯基-1-苦基肼（DPPH）自由基清除和还原能力测定评估抗氧化活性。使用细胞活力、凋亡、细胞增殖和细胞周期测定来评估对HeLa细胞的细胞毒性潜力。白藜芦醇苷和松 stilbenoside 作为自由基清除剂和还原剂表现出较低的活性。然而，它们在降低人宫颈癌HeLa细胞的活力和抑制增殖方面显示出更大的功效。鉴于我们研究的有希望的发现，应进一步研究白藜芦醇苷和松 stilbenoside 的治疗潜力。
