Université d'Aix-Marseille, 13108 Saint-Paul-lez-Durance, France.
CNRS, UMR 7265, 13108 Saint-Paul-lez-Durance, France.
Int J Syst Evol Microbiol. 2013 Jul;63(Pt 7):2600-2606. doi: 10.1099/ijs.0.048454-0. Epub 2012 Dec 21.
An actinobacterial strain, designated ViU22(T), was isolated from a natural uranium-rich soil and was studied using a polyphasic approach. Cells formed orange-pigmented colonies, were rod-shaped, Gram-positive (non-staining method), non-motile and non-spore-forming. This organism grew in 0-4.5 % (w/v) NaCl and at 15-37 °C, with optimal growth occurring in 0.5 % (w/v) NaCl and at 30 °C. Comparative 16S rRNA gene sequence analysis revealed that the strain ViU22(T) belonged to the genus Microbacterium. It exhibited highest 16S rRNA gene sequence similarity with the type strains of Microbacterium testaceum (98.14 %) and Microbacterium binotii (98.02 %). The DNA-DNA relatedness of strains ViU22(T) with the most closely related type strains Microbacterium testaceum and Microbacterium binotii DSM 19164(T) was 20.10 % (± 0.70) and 28.05 % (± 0.35), respectively. Strain ViU22(T) possessed a type B2β peptidoglycan with partial substitution of glutamic acid by 3-hydroxy glutamic acid. The major menaquinones were MK-11 and MK-12. Major polar lipids detected in the strain ViU22(T) were diphosphatidylglycerol, phosphatidylglycerol, an unknown phospholipid and unknown glycolipids. The predominant fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0, a pattern reported for other Microbacterium species. The major cell-wall sugars were galactose, xylose and mannose and the DNA G+C content was 71 mol%. Together, the DNA-DNA hybridization results and the differentiating phenotypic characteristics, showed that strain ViU22(T) should be classified as the type strain of a novel species within the genus Microbacterium, for which the name Microbacterium lemovicicum sp. nov. is proposed. The type strain is ViU22(T) ( = ATCC BAA-2396(T) = CCUG 62198(T) = DSM 25044(T)).
一株放线菌，命名为 ViU22(T)，从富含天然铀的土壤中分离得到，采用多相分类法进行研究。细胞形成橙色色素菌落，呈杆状，革兰氏阳性（非染色法），不运动，不形成孢子。该菌在 0-4.5%（w/v）NaCl 和 15-37°C 下生长，最佳生长条件为 0.5%（w/v）NaCl 和 30°C。比较 16S rRNA 基因序列分析表明，菌株 ViU22(T)属于微杆菌属。它与模式菌株嗜甲基微杆菌（98.14%）和双生微杆菌（98.02%）的 16S rRNA 基因序列相似度最高。菌株 ViU22(T)与最密切相关的模式菌株嗜甲基微杆菌和双生微杆菌 DSM 19164(T)的 DNA-DNA 同源性分别为 20.10%（±0.70）和 28.05%（±0.35）。菌株 ViU22(T)具有 B2β 型肽聚糖，部分谷氨酸被 3-羟基谷氨酸取代。主要的类异戊二烯醌是 MK-11 和 MK-12。在菌株 ViU22(T)中检测到的主要极性脂是双磷脂酰甘油、磷脂酰甘油、一种未知的磷脂和未知的糖脂。主要脂肪酸为anteiso-C15:0、anteiso-C17:0 和 iso-C16:0，这是其他微杆菌属物种的特征。主要细胞壁糖为半乳糖、木糖和甘露糖，DNA G+C 含量为 71mol%。综合 DNA-DNA 杂交结果和区分表型特征，表明菌株 ViU22(T)应归类为微杆菌属的一个新种的模式株，建议将其命名为 lemovicicum 微杆菌新种。模式菌株为 ViU22(T)=ATCC BAA-2396(T)=CCUG 62198(T)=DSM 25044(T)。
