Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Beijing 100081, PR China.
Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, PR China.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):236-240. doi: 10.1099/ijs.0.038166-0. Epub 2012 Mar 23.
A Gram-positive, non-motile, non-spore-forming, rod-shaped bacterium, strain 7087(T), was isolated from rhizosphere of maize in China. The strain grew at 4-50 °C and at pH 4-10, with optima of 37 °C and pH 7.0, respectively. Phylogenetic analysis based on the full-length 16S rRNA gene sequence revealed that strain 7087(T) was a member of the genus Microbacterium. High levels of 16S rRNA gene similarities were found between strain 7087(T) and Microbacterium binotii DSM 19164(T) (99.8 %). However, the DNA-DNA hybridization value between strain 7087(T) and Microbacterium binotii DSM 19164(T) was 24.2 %. The DNA G+C content of strain 7087(T) was 69.9 mol%. The major fatty acids were anteiso-C(17 : 0) (36.45 %), anteiso-C(15 : 0) (36.08 %) and iso-C(16 : 0) (16.11 %). The predominant menaquinones were MK-10 (28.1 %), MK-11 (54.8 %) and MK-12 (17.1 %). The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine. The major polar lipids are diphosphatidylglycerol, unknown phospholipids, an unknown glycolipid and unknown amino lipids. On the basis of these results, strain 7087(T) is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium neimengense sp. nov. is proposed. The type strain is 7087(T) ( = ACCC 03008(T) = DSM 24985(T)).
一株革兰氏阳性、非运动、非孢子形成、杆状细菌，菌株 7087(T)，从中国玉米根际中分离得到。该菌株在 4-50°C 和 pH4-10 的条件下生长，最适生长温度为 37°C，最适 pH 值为 7.0。基于全长 16S rRNA 基因序列的系统发育分析表明，菌株 7087(T)是微杆菌属的一个成员。菌株 7087(T)与 Microbacterium binotii DSM 19164(T)的 16S rRNA 基因相似度高达 99.8%。然而，菌株 7087(T)与 Microbacterium binotii DSM 19164(T)之间的 DNA-DNA 杂交值为 24.2%。菌株 7087(T)的 DNA G+C 含量为 69.9mol%。主要脂肪酸为 anteiso-C(17:0) (36.45%)、anteiso-C(15:0) (36.08%)和 iso-C(16:0) (16.11%)。主要的menaquinones 为 MK-10 (28.1%)、MK-11 (54.8%)和 MK-12 (17.1%)。细胞壁肽聚糖中的二氨基氨基酸为鸟氨酸。主要的极性脂为双磷脂酰甘油、未知磷脂、未知糖脂和未知氨基脂。基于这些结果，菌株 7087(T)被认为代表了微杆菌属的一个新种，命名为 Neimengensimicrobacterium neimengense sp. nov.。模式菌株为 7087(T)（=ACCC 03008(T)=DSM 24985(T)）。
