Yan Zheng-Fei, Lin Pei, Won Kyung-Hwa, Yang Jung-Eun, Li Chang-Tian, Kook MooChang, Wang Qi-Jun, Yi Tae-Hoo
Department of Oriental Medicinal Material and Processing, College of Life Sciences, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do, 17104, Republic of Korea.
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3564-3569. doi: 10.1099/ijsem.0.002167. Epub 2017 Sep 6.
A Gram-stain-positive, aerobic, non-motile, short-rod shaped actinobacterium, designated THG-T2.14T, was isolated from soil sampled from the rhizosphere of mugunghwa. Growth occurred at 10-40 °C (optimum 30 °C), at pH 5.0-10.0 (optimum 7.0) and at 0-7.0 % NaCl (optimum 3.0 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T2.14T were identified as Microbacterium yannicii DSM 23203T (98.8 %), Microbacterium trichothecenolyticum DSM 8608T (98.8 %), Microbacterium arthrosphaerae DSM 22421T (98.7 %) and Microbacterium jejuense KACC 17124T (98.4 %). The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unidentified lipid, two unidentified phospholipids and two unidentified phosphoglycolipids. The menaquinones were MK-12, and MK-13. The major polyamine was spermidine. The peptidoglycan contained ornithine, alanine, glycine, homoserine and glutamic acid. The diagnostic diamino acid was ornithine. The acyl type of the muramic acid was glycolyl. The whole-cell sugars were rhamnose, ribose, galactose, arabinose, xylose and glucose. The DNA G+C content of strain THG-T2.14T was 71.2 mol%. The DNA-DNA relatedness between strain THG-T2.14T and its closest reference strains were significantly lower than the threshold value of 70 %. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T2.14T represents a novel species of the genus Microbacterium, for which the name Microbacterium hibisci sp. nov. is proposed. The type strain is THG-T2.14T (=KACC 18931T=CCTCC AB 2016180T).
从木槿根际土壤中分离出一株革兰氏染色阳性、需氧、不运动、短杆状的放线菌，命名为THG-T2.14T。该菌在10-40°C（最适温度30°C）、pH 5.0-10.0（最适pH 7.0）和0-7.0% NaCl（最适盐浓度3.0%）条件下生长。基于16S rRNA基因序列分析，菌株THG-T2.14T最近的系统发育邻居被鉴定为扬尼氏微小杆菌DSM 23203T（相似度98.8%）、解单端孢霉烯微小杆菌DSM 8608T（相似度98.8%）、节球微小杆菌DSM 22421T（相似度98.7%）和济州微小杆菌KACC 17124T（相似度98.4%）。主要脂肪酸为anteiso-C15:0、anteiso-C17:0和iso-C16:0。极性脂为双磷脂酰甘油、磷脂酰甘油、一种未鉴定的脂类、两种未鉴定的磷脂和两种未鉴定的磷酸糖脂。甲基萘醌为MK-12和MK-13。主要多胺为亚精胺。肽聚糖含有鸟氨酸、丙氨酸、甘氨酸、高丝氨酸和谷氨酸。诊断性二氨基酸为鸟氨酸。胞壁酸的酰基类型为糖基。全细胞糖类为鼠李糖、核糖、半乳糖、阿拉伯糖、木糖和葡萄糖。菌株THG-T2.14T的DNA G+C含量为71.2 mol%。菌株THG-T2.14T与其最接近的参考菌株之间的DNA-DNA相关性显著低于70%的阈值。基于系统发育分析、化学分类数据、生理特征和DNA-DNA杂交数据，菌株THG-T2.14T代表微小杆菌属的一个新物种，为此提出名为木槿微小杆菌（Microbacterium hibisci sp. nov.）的名称。模式菌株为THG-T2.14T（=KACC 18931T=CCTCC AB 2016180T）。
