Lucio B, Hitchner S B
Avian Dis. 1979 Oct-Dec;23(4):1037-50.
Modified infectious bursal disease virus (IBDV) administered ocularly to either susceptible or passively immune chicks did not induce protection against bursal atrophy by wild IBDV, while intramuscular (IM) or intrabursal (IB) injection protected susceptible chickens. No protection was obtained in passively immune chickens vaccinated IM or IB. Susceptible and passively immune chickens vaccinated with a single dose of killed-virus suspension (KVS) did not become resistant to wild IBDV challenge. A killed-virus multiple emulsion (KVME) induced partial protection (3/5) against challenge 28 days after subcutaneous vaccination of three-day-old susceptible chickens. At 35 and 42 days old, all susceptible chickens were protected by KVME. Protection by passive antibodies was observed in unvaccinated, KVS-, and KVME-vaccinated chickens challenged at 10 and 17 days of age. Protection was only partial in passively immune chickens vaccinated with KVME after passive antibodies were no longer protective to unvaccinated and KVS-vaccinated chicks. Modified IBDV was detected two days post-IB inoculation only in the bursa of Fabricius of susceptible chickens, while wild IBDV was found also in the thymus, spleen, and blood. No evidence of virus was found in immune chickens inoculated IB with modified IBDV. The growth of wild IBDV was limited to the bursa of Fabricius in immune chickens. Furthermore, the intensity of fluorescence, as well as the number of positive bursa cells, was low when compared with fluorescence in the bursa of susceptible chickens infected with wild IBDV.
将改良型传染性法氏囊病病毒（IBDV）经眼接种易感或被动免疫的雏鸡后，不能诱导其对野生IBDV引起的法氏囊萎缩产生保护作用，而肌肉注射（IM）或法氏囊内注射（IB）则可保护易感鸡。对经IM或IB接种疫苗的被动免疫鸡未获得保护作用。用单剂量灭活病毒悬液（KVS）接种的易感和被动免疫鸡对野生IBDV攻击未产生抵抗力。在对3日龄易感鸡进行皮下接种28天后，灭活病毒多重乳剂（KVME）诱导了部分保护作用（5只中有3只）。在35日龄和42日龄时，所有易感鸡均受到KVME的保护。在10日龄和17日龄受到攻击的未接种疫苗、接种KVS和接种KVME的鸡中观察到被动抗体的保护作用。在被动抗体对未接种疫苗和接种KVS的雏鸡不再具有保护作用后，用KVME接种的被动免疫鸡的保护作用仅为部分保护。仅在易感鸡的法氏囊中于IB接种后两天检测到改良型IBDV，而在胸腺、脾脏和血液中也发现了野生IBDV。在经IB接种改良型IBDV的免疫鸡中未发现病毒迹象。野生IBDV的生长仅限于免疫鸡的法氏囊。此外，与感染野生IBDV的易感鸡法氏囊中的荧光相比，荧光强度以及阳性法氏囊细胞数量均较低。
