Guo Xiaochen, Sun Wenying, Hu Changhui, Wang Xiangxiang, Li Shuang, Qin Ruihan, Wang Yixuan, Liu Jinmiao, Xue Zhiqiang, Li Huijuan, Zhang Tingting, Wang Wei, Guo Yunpeng, Yin Jiechao, Jiang Ming, Zhao Han, Wei Lan, Zhang Jingmin, Ren Guiping
Biopharmaceutical Lab, College of Life Science, Northeast Agricultural University, Harbin 150030, China.
Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin 150010, China.
Transbound Emerg Dis. 2023 Jun 28;2023:5904280. doi: 10.1155/2023/5904280. eCollection 2023.
Newcastle disease (ND) and infectious bursal disease (IBD) are both one of the most economically important infectious diseases, which cause high infection rate and mortality of chickens and restrict the development of the poultry industry. Currently, vaccination is the most effective method to prevent ND and IBD. In this study, the biological adjuvant bivalent vaccine (rClone30-VP2L-chGM-CSF) was developed for preventing ND and IBD. Furthermore, the immune and protective effects of rClone30-VP2L-chGM-CSF were evaluated in specific-pathogen-free chickens. The experimental results indicated that chickens immunized with rClone30-VP2L-chGM-CSF vaccine significantly improved anti-IBDV and anti-NDV antibody titer; the level of CD4 T, CD8 T, B, MHC-II, and monocyte/macrophagocyte+ cells; the concentrations of IL-1, IL-4, IL-17, IFN-, IFN-, and IFN-; and the splenocyte proliferation rate. Anti-NDV antibody titer reached the theoretical protection value of 4log2 at 7 days after immunization in chickens of rClone30-VP2L-chGM-CSF group; however, which at 14 days after immunization in chickens of rClone30-VP2L and rClone30. These results showed that chickens immunized with rClone30-VP2L-chGM-CSF stimulated stronger immune response than those with the rClone30-VP2L and rClone30. Chickens were challenged with virulent IBDV BC6/85, which were protected in the rClone30-VP2L-chGM-CSF group. Furthermore, IBDV RNA was not measured, and there appeared to be little apoptosis in the bursa of Fabricius in the rClone30-VP2L-chGM-CSF group. Therefore, rClone30-VP2L-chGM-CSF is a promising vaccine candidate against infectious bursal disease virus (IBDV) and newcastle disease virus (NDV), and it provides an idea for developing other poultry vaccines.
新城疫（ND）和传染性法氏囊病（IBD）都是经济上最重要的传染病之一，它们导致鸡的高感染率和死亡率，并限制了家禽业的发展。目前，接种疫苗是预防新城疫和传染性法氏囊病最有效的方法。在本研究中，开发了用于预防新城疫和传染性法氏囊病的生物佐剂二价疫苗（rClone30-VP2L-chGM-CSF）。此外，在无特定病原体的鸡中评估了rClone30-VP2L-chGM-CSF的免疫和保护作用。实验结果表明，用rClone30-VP2L-chGM-CSF疫苗免疫的鸡显著提高了抗传染性法氏囊病病毒（IBDV）和抗新城疫病毒（NDV）抗体滴度；CD4 T细胞、CD8 T细胞、B细胞、MHC-II以及单核细胞/巨噬细胞+细胞的水平；白细胞介素-1（IL-1）、白细胞介素-4（IL-4）、白细胞介素-17（IL-17）、干扰素-γ（IFN-γ）、干扰素-α（IFN-α）和干扰素-β（IFN-β）的浓度；以及脾细胞增殖率。rClone30-VP2L-chGM-CSF组鸡在免疫后7天抗新城疫病毒抗体滴度达到理论保护值4log2；然而，rClone30-VP2L组和rClone30组鸡在免疫后14天才达到。这些结果表明，用rClone30-VP2L-chGM-CSF免疫的鸡比用rClone30-VP2L和rClone30免疫的鸡刺激产生更强的免疫反应。用强毒传染性法氏囊病病毒BC6/85攻击鸡，rClone30-VP2L-chGM-CSF组鸡受到保护。此外，未检测到传染性法氏囊病病毒RNA，rClone30-VP2L-chGM-CSF组法氏囊中似乎几乎没有细胞凋亡。因此，rClone30-VP2L-chGM-CSF是一种有前景的抗传染性法氏囊病病毒（IBDV）和新城疫病毒（NDV）的候选疫苗，它为开发其他家禽疫苗提供了思路。
