Gershbein L L
Northwest Institute for Medical Research, Chicago, IL 60634.
Res Commun Chem Pathol Pharmacol. 1990 Jan;67(1):163-6.
Groups of mature female Syrian golden hamsters were injected s.c. with peanut oil solutions of estradiol benzoate and progesterone at daily dosages of 10 micrograms and 20 mg, respectively, the controls receiving the vehicle. The male series comprised controls and estrogen-treated. A portion of each group was injected i.p. with phenobarbital at 80 mg/kg daily for the last 3 days and all animals were sacrificed on day 10. Hepatic microsomal total protein, cytochrome P-450 and enzymes, aminopyrine demethylase and benzo[a]pyrene hydroxylase of the steroid-injected groups revealed no definite changes over the controls except for an increase in the hydroxylase activity with the progesterone group. In general, cytochrome P-450 and the enzyme activities were significantly elevated on comparison of the phenobarbital-injected groups versus the uninduced controls. Inter-group comparisons of the phenobarbital-injected females revealed no remarkable changes over the respective controls, but with the males, the hydroxylase activity of the estrogen-treated group was markedly elevated. In contrast to the rat, the hamsters injected with estrogen at the specified dosage, underwent no significant change in the liver weight percentages. However, liver enlargement occurred with progesterone as such or on phenobarbital treatment in comparison with the corresponding control groups.
将成年雌性叙利亚金黄地鼠分组，分别皮下注射苯甲酸雌二醇和孕酮的花生油溶液，每日剂量分别为10微克和20毫克，对照组注射溶媒。雄性系列包括对照组和雌激素处理组。每组的一部分在最后3天每天腹腔注射80毫克/千克苯巴比妥，所有动物在第10天处死。除孕酮组的羟化酶活性增加外，注射类固醇组的肝微粒体总蛋白、细胞色素P - 450和酶、氨基比林脱甲基酶和苯并[a]芘羟化酶与对照组相比无明显变化。一般来说，与未诱导的对照组相比，注射苯巴比妥的组中细胞色素P - 450和酶活性显著升高。注射苯巴比妥的雌性组间比较显示，与各自对照组相比无明显变化，但雄性中，雌激素处理组的羟化酶活性显著升高。与大鼠不同，以指定剂量注射雌激素的地鼠肝脏重量百分比无显著变化。然而，与相应对照组相比，单独使用孕酮或经苯巴比妥处理后肝脏会肿大。
