White A C, Gershbein L L
Res Commun Chem Pathol Pharmacol. 1987 Mar;55(3):317-34.
Rats of either sex as intact or partially hepatectomized (two-thirds liver removal) were injected s.c. daily for 7 days post-operatively with natural and synthetic estrogens, androgens or anabolic steroids, progesterone and adrenal cortical hormones and killed on day 10 at which time the livers were processed for microsomal analyses (protein, cytochrome P-450 and enzymes, aminopyrine demethylase and aromatic hydrocarbon or benzo[a]pyrene hydroxylase). Groups were also induced with phenobarbital injected i.p. on the last 3 days at 80 mg/kg each. With the intact males, hexestrol was the only estrogen which caused liver enlargement and at a daily dosage of 1.0 micrograms per rat as well as a decrease in the hydroxylase level. Estradiol benzoate (15 micrograms/rat daily) depressed cytochrome P-450 and the other steroids screened had little effect on the microsomal parameters except for a rise in demethylase with deoxycorticosterone acetate (1.0 mg). A greater sensitivity to estrogens and the other steroid types was noted with the partially hepatectomized males and in the direction of depressions in the microsomal elements, the respective data being expressed as percentages of the controls. Estrogens were better tolerated by the intact female and in general, liver enlargement was remarkable. Microsomal activity was elevated with estradiol benzoate or little affected by estrogens except for depressions in hydroxylase levels with equilin (15 micrograms) and the high dosages of hexestrol and diethylstilbestrol, the last agent also eliciting an increase in cytochrome P-450. No statistically significant effect on the microsomal parameters was observed with the intact female treated with 17-methyltestosterone and the anabolic steroids but decreases occurred on injection with testosterone propionate and the cortical hormones. As with the operated male, partially hepatectomized females exhibited no hepatotrophic response to estrogens and the microsomal changes were moderate with such agents as with the other steroid types and resembling the findings for intact males. Competitive inhibition was followed by Lineweaver-Burke analyses of aminopyrine demethylase in operated females treated with several of the steroids.
将完整的或部分肝切除（切除三分之二肝脏）的雌雄大鼠，在术后每天皮下注射天然和合成雌激素、雄激素、合成代谢类固醇、孕酮和肾上腺皮质激素，持续7天，并在第10天处死，此时对肝脏进行微粒体分析（蛋白质、细胞色素P - 450和酶、氨基比林脱甲基酶以及芳烃或苯并[a]芘羟化酶）。在最后3天，每组还腹腔注射苯巴比妥，剂量为80毫克/千克。对于完整的雄性大鼠，己烯雌酚是唯一导致肝脏肿大的雌激素，剂量为每只大鼠每天1.0微克，同时羟化酶水平降低。苯甲酸雌二醇（每天15微克/大鼠）降低细胞色素P - 450，除了醋酸脱氧皮质酮（1.0毫克）使脱甲基酶升高外，其他筛选的类固醇对微粒体参数影响很小。部分肝切除的雄性大鼠对雌激素和其他类固醇类型更为敏感，微粒体成分呈降低趋势，各自的数据表示为对照的百分比。完整的雌性大鼠对雌激素耐受性更好，总体上肝脏肿大明显。苯甲酸雌二醇使微粒体活性升高，或除了马萘雌酮（15微克）、高剂量己烯雌酚和己烯雌酚使羟化酶水平降低外，雌激素对其影响很小，但己烯雌酚也使细胞色素P - 450增加。用17 - 甲基睾酮和合成代谢类固醇处理完整的雌性大鼠，对微粒体参数未观察到统计学上的显著影响，但注射丙酸睾酮和皮质激素后出现降低。与手术雄性大鼠一样，部分肝切除的雌性大鼠对雌激素无肝营养反应，微粒体变化与其他类固醇类型相似，且与完整雄性大鼠的结果相似。对用几种类固醇处理的手术雌性大鼠的氨基比林脱甲基酶进行Lineweaver - Burke分析，结果显示存在竞争性抑制。
