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STA1，一个拟南芥前体 mRNA 加工因子 6 同源物，是一个新的参与 miRNA 生物发生的因子。

STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis.

机构信息

Department of Plant Biology and Biotechnology, Faculty of Life Science, University of Copenhagen, Thovanlsensvej 40, 1871 Frederiksberg, Copenhagen, Denmark.

出版信息

Nucleic Acids Res. 2013 Feb 1;41(3):1984-97. doi: 10.1093/nar/gks1309. Epub 2012 Dec 24.

DOI:10.1093/nar/gks1309
PMID:23268445
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3561960/
Abstract

MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processing of primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesis-defective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.

摘要

microRNAs (miRNAs) 是一类在真核生物中参与多种发育和代谢过程的重要调控小 RNA。在拟南芥中，DICER-LIKE1（DCL1）、HYPNASTIC LEAVES 1（HYL1）、SERRATE（SE）、HUA ENHANCER1（HEN1）和 HASTY 参与初级 miRNA（pri-miRNA）加工为前体 miRNA（pre-miRNA），最终产生 miRNA。除了这些成分外，mRNA 帽结合蛋白 CBP80/ABA HYPERSENSITIVE1（CBP80/ABA HSP）和 CBP20 也参与 miRNA 的生物发生。在这里，我们表明，拟南芥前体 mRNA 加工因子 6 同源物 STABILIZED1（STA1）也参与 miRNA 的生物发生。与其他 miRNA 生物发生缺陷突变体相似，sta1-1 积累的成熟 miRNA 水平明显低于野生型，同时积累的 pri-miRNA 水平高于野生型。成熟 miRNA 的显著减少与靶基因转录物的积累和发育缺陷有关。此外，sta1-1 还干扰了含有内含子的 pri-miRNA 的剪接，并降低了 DCL1 的转录水平。这些结果表明，STA1 通过参与 pri-miRNA 的剪接直接参与 miRNA 的生物发生，通过调节 DCL1 的转录水平间接参与 miRNA 的生物发生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/47c52debab1f/gks1309f7p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/e45d30587e0a/gks1309f1p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/e3dcd9687702/gks1309f2p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/303a39ca7cac/gks1309f3p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/a766706e9570/gks1309f4p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/5a5b7de98747/gks1309f5p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/7ab2ba5fc565/gks1309f6p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/47c52debab1f/gks1309f7p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/e45d30587e0a/gks1309f1p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/e3dcd9687702/gks1309f2p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/303a39ca7cac/gks1309f3p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/a766706e9570/gks1309f4p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/5a5b7de98747/gks1309f5p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/7ab2ba5fc565/gks1309f6p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f02/3561960/47c52debab1f/gks1309f7p.jpg

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