Department of Bionanotechnology, Gachon University, Gyeonggi-Do.
Int J Nanomedicine. 2012;7:6123-36. doi: 10.2147/IJN.S38402. Epub 2012 Dec 18.
A novel clinical glucose biosensor fabricated using functionalized metalloid-polymer (silver-silica coated with polyethylene glycol) hybrid nanoparticles on the surface of a graphene oxide nanosheet is reported. The cyclic voltammetric response of glucose oxidase modification on the surface of a functionalized graphene oxide electrode showed a surface-confined reaction and an effective redox potential near zero volts, with a wide linearity of 0.1-20 mM and a sensitivity of 7.66 μA mM(-1) cm(-2). The functionalized graphene oxide electrode showed a better electrocatalytic response toward oxidation of H(2)O(2) and reduction of oxygen. The practical applicability of the functionalized graphene oxide electrode was demonstrated by measuring the peak current against multiple urine and serum samples from diabetic patients. This new hybrid nanoarchitecture combining a three-dimensional metalloid-polymer hybrid and two-dimensional graphene oxide provided a thin solid laminate on the electrode surface. The easy fabrication process and retention of bioactive immobilized enzymes on the functionalized graphene oxide electrode could potentially be extended to detection of other biomolecules, and have broad applications in electrochemical biosensing.
一种新型临床葡萄糖生物传感器，使用功能化的类金属聚合物（银-二氧化硅涂覆的聚乙二醇）杂化纳米粒子在氧化石墨烯纳米片的表面上制造。葡萄糖氧化酶在功能化石墨烯电极表面上的修饰的循环伏安响应显示表面限制的反应和接近零伏的有效氧化还原电位，具有 0.1-20 mM 的宽线性度和 7.66 μA mM(-1) cm(-2)的灵敏度。功能化的石墨烯电极对 H(2)O(2)的氧化和氧气的还原表现出更好的电催化响应。通过测量来自糖尿病患者的多个尿液和血清样本的峰值电流，证明了功能化的石墨烯电极的实际适用性。这种将三维类金属聚合物杂化和二维石墨烯氧化物结合在一起的新型杂化纳米结构在电极表面提供了一个薄的固体层压板。功能化的石墨烯电极上易于制造过程和保留生物活性固定化酶的方法可能会扩展到其他生物分子的检测，并在电化学生物传感中有广泛的应用。
