超高效液相色谱法同时测定炭茜草中4种醌类化合物

[Simultaneous determination of four quinone compounds in carbonized Rubiae Radix et Rhizoma by UPLC].

作者信息

Chen Xing, Wang Kan, Shan Ming-qiu, Ding An-wei

机构信息

Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2012 Oct;37(19):2922-5.

PMID:23270235

Abstract

OBJECTIVE

To establish an UPLC method for simultaneous determination of purpuroxanthine, purpurin, 1,3,6-trihydroxy-2-methylanthraquinone, rubimaillin in carbonized Rubiae Radix et Rhizoma.

METHOD

The components were separated on acquity BEHC18 (2.1 mm x 50 mm, 1.7 microm) using methanol and 0.3% formic acid solution as the mobile phase; The flow rate was 0.2 mL x min(-1) and the volume of injection was 2 microL; the column temperature was maintained at 30 degrees C and the detective wavelength was set at 276 nm.

RESULT

There were good liner relationships between the peak area and concentration at ranges of 0.68-34.44 mg x L(-1) (r = 0.9999), 0.66-33.2 mg x L(-1) (r = 0.9997), 0.68-34.08 mg x L(-1) (r = 0.9999), 1.07-53.52 mg x L(-1) (r = 0.9999) for purpuroxanthine, purpurin, 1,3,6-trihydroxy-2-methylanthraquinone, rubimaillin, respectively; the average recovery rates of purpuroxanthine, purpurin, 1,3,6-trihydroxy-2-methylanthraquinone, rubimaillin were 96.95%, 95.75%, 102.5%, 96.15%, respectively, with RSD less than 3%.

CONCLUSION

The established method was rapid and simple with good accuracy and reproducibility for the determination of carbonized Rubiae Radix et Rhizoma, the method was suitable for the quality control of carbonized Rubiae Radix et Rhizoma.

摘要

目的

建立超高效液相色谱法同时测定焦茜草中紫黄嘌呤、紫红素、1,3,6 - 三羟基 - 2 - 甲基蒽醌、茜草素的含量。

方法

采用acquity BEHC18（2.1 mm×50 mm，1.7μm）色谱柱，以甲醇和0.3%甲酸溶液为流动相进行分离；流速为0.2 mL·min⁻¹，进样量为2μL；柱温保持在30℃，检测波长设定为276 nm。

结果

紫黄嘌呤、紫红素、1,3,6 - 三羟基 - 2 - 甲基蒽醌、茜草素分别在0.68 - 34.44 mg·L⁻¹（r = 0.9999）、0.66 - 33.2 mg·L⁻¹（r = 0.9997）、0.68 - 34.08 mg·L⁻¹（r = 0.9999）、1.07 - 53.52 mg·L⁻¹（r = 0.9999）范围内峰面积与浓度呈良好的线性关系；紫黄嘌呤、紫红素、1,3,6 - 三羟基 - 2 - 甲基蒽醌、茜草素的平均回收率分别为96.95%、95.75%、102.5%、96.15%，相对标准偏差均小于3%。