[碳化茜草（cRRR）不同极性部位对氧化损伤的保护作用]

[Protective effect of different polar fractions of Carbonized Rubiae Radix et Rhizoma (cRRR) against oxidative injury].

作者信息

Xiao Lin-Yan, Wang Hai-Li, Chen Yi, Zhang Li, Cheng Fang-Fang, Shan Ming-Qiu, Ding An-Wei

机构信息

National and Local United Engineering Research Center for Traditional Chinese Medicine Resource Industrialization and Prescription Innovation Medicine, Jiangsu Collaborative Innovation Center of Traditional Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2017 Dec;42(23):4604-4610. doi: 10.19540/j.cnki.cjcmm.20171023.007.

DOI:10.19540/j.cnki.cjcmm.20171023.007

PMID:29376259

Abstract

The protective effect of different polar fractions of Carbonized Rubiae Radix et Rhizoma (cRRR) against ox-LDL-induced damage to human umbilical vein endothelial cells (HUVECs) was investigated by MTT assay, and the components were identified by using UPLC-Q-TOF-MS. According to the study, ethyl acetate extract and n-butanol extract could increase cell viability (P<0.01), while petroleum ether extract had no influence, and water extract could even inhibit the cell viability to some degree. Moreover, 32 compounds in four polar fractions were analyzed, including 31 quinones and their glycosides, and one rubiprasins C. Petroleum ether extract, ethyl acetate extract, n-butanol extract and water extract contained 23, 32, 26, 15 compounds, respectively. According to cell experiments in vitro, active fractions were ethyl acetate extract and n-butanol extract. The results could provide scientific references for further studies on effective material basic of cRRR, and lay a foundation for studies on the relationship between efficacies and materials.

摘要

采用MTT法研究了茜草炭不同极性部位对氧化型低密度脂蛋白（ox-LDL）诱导的人脐静脉内皮细胞（HUVECs）损伤的保护作用，并通过超高效液相色谱-四极杆飞行时间质谱联用仪（UPLC-Q-TOF-MS）对其成分进行鉴定。研究表明，乙酸乙酯提取物和正丁醇提取物可提高细胞活力（P<0.01），而石油醚提取物无影响，水提取物甚至在一定程度上抑制细胞活力。此外，对四个极性部位的32种化合物进行了分析，包括31种醌类及其糖苷，以及1种茜草素C。石油醚提取物、乙酸乙酯提取物、正丁醇提取物和水提取物分别含有23、32、26、15种化合物。体外细胞实验表明，活性部位为乙酸乙酯提取物和正丁醇提取物。该结果可为进一步研究茜草炭有效物质基础提供科学参考，为药效与物质关系的研究奠定基础。