Dept of Nephrology, State Key Lab of Kidney Diseases, Chinese PLA General Hospital, Beijing, China.
Am J Clin Pathol. 2013 Jan;139(1):71-8. doi: 10.1309/AJCPRZG8EXN7BAID.
Immunofluorescence of frozen tissue sections (IF-F) is a classic technique for renal immunopathologic examination. However, it has certain disadvantages, such as diffuse antigen distribution and few or even no glomeruli in the section. We developed a new technique of immunofluorescence staining using dual microwave retrieval in paraffin-embedded renal tissue sections (IF-DMP) and compared IF-DMP with IF-F in 406 renal biopsy samples. IF-DMP detected significantly more glomeruli than did IF-F (P< .001). There was no significant difference for the specificity and sensitivity in the detection of immunoglobulins, complements, κ, and λ between IF-F and IF-DMP. Concordant observations were 98% for all immunofluorescence, complements, κ, and λ staining and 100% for immunoglobulin staining. Both techniques were completely accurate in confirming diagnoses of various glomerular diseases. IF-DMP provided clearer images of tissue structure and more precise localization of antigens, and it is a suitable alternative for traditional IF-F in clinical renal immunopathologic diagnosis.
免疫荧光冰冻组织切片检查（IF-F）是肾脏免疫病理检查的经典技术。然而，它有一定的缺点，如抗原弥散分布和切片中肾小球数量少甚至没有。我们开发了一种新的免疫荧光染色技术，即使用双重微波在石蜡包埋的肾组织切片中进行检索（IF-DMP），并将 IF-DMP 与 406 例肾活检样本中的 IF-F 进行比较。IF-DMP 检测到的肾小球数量明显多于 IF-F（P<.001）。IF-F 和 IF-DMP 在检测免疫球蛋白、补体、κ 和 λ 方面的特异性和敏感性无显著差异。所有免疫荧光、补体、κ 和 λ 染色的一致性观察为 98%，免疫球蛋白染色的一致性观察为 100%。两种技术在确认各种肾小球疾病的诊断方面均完全准确。IF-DMP 提供了更清晰的组织结构图像和更精确的抗原定位，是临床肾脏免疫病理诊断中传统 IF-F 的合适替代方法。
