荧光免疫组织化学。

Fluorescent Immunohistochemistry.

机构信息

Arkana Laboratories, Little Rock, AR, USA.

Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.

出版信息

Methods Mol Biol. 2022;2422:131-146. doi: 10.1007/978-1-0716-1948-3_9.

DOI:10.1007/978-1-0716-1948-3_9

PMID:34859403

Abstract

Immunofluorescence is an important immunochemical technique that utilizes fluorescence-labeled antibodies to detect specific target antigens. It is used widely in both scientific research and clinical laboratories. Immunofluorescence allows for excellent sensitivity and amplification of signal in comparison to immunohistochemistry. However, analysis of samples labeled with fluorescence-labeled antibodies has to be performed using a fluorescence microscope or other type of fluorescence imaging. There are two methods available: direct (primary) and indirect (secondary) immunofluorescence. Here, we describe the principle of immunofluorescence methods as well as the preparation of fresh-frozen and formalin-fixed, paraffin embedded tissues for both direct and indirect immunofluorescence labeling.

摘要

免疫荧光是一种重要的免疫化学技术，利用荧光标记的抗体来检测特定的靶抗原。它在科学研究和临床实验室中都得到了广泛的应用。与免疫组织化学相比，免疫荧光具有更好的灵敏度和信号放大效果。然而，用荧光标记的抗体标记的样品分析必须使用荧光显微镜或其他类型的荧光成像进行。有两种方法可用：直接（初级）和间接（次级）免疫荧光。在这里，我们描述了免疫荧光方法的原理以及为直接和间接免疫荧光标记准备新鲜冷冻和福尔马林固定、石蜡包埋组织的方法。