Alwahaibi Nasar Yousuf, Alsidiri Rajaa Mohammed, Alsinawi Thuraiya Amer, Almalki Wafa Hamed, Alsinawi Shadia Said, Alriyami Marwa Abdullah
Department of Allied Health Sciences, College of Medicine and Health Sciences, Sultan Qaboos University, Sultanate of Oman.
Department of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Sultanate of Oman.
Indian J Nephrol. 2020 Jan-Feb;30(1):8-13. doi: 10.4103/ijn.IJN_356_18. Epub 2019 Dec 27.
There are few published studies comparing immunofluorescence on formalin-fixed, paraffin-embedded (FFPE) tissue sections (IF-P) and immunoperoxidase on FFPE tissue sections (IP-P) with immunofluorescence on frozen sections (IF-F) for evaluation of renal diseases. Also, the accuracy for each method differs greatly. The aim of this study was to evaluate IF-P and IP-P as an alternative to IF-F (gold standard method) in the diagnosis of renal biopsies specimens.
In all, 101 renal biopsies were subjected to IF-P, IP-P, and IF-F staining to demonstrate immunoglobulin IgA, IgG, and IgM immune deposits. Sensitivity, specificity, false-positive, and false-negative values were calculated.
IP-P showed sensitivity of 61.8%, 74.2%, and 64.2%, and specificity of 84.8%, 69.2%, and 66.7% for IgA, IgG, and IgM, respectively. IF-P showed a sensitivity of 45.6%, 69.4% and 52.8%, and specificity of 87.9%, 87.2% and 77.1% for IgA, IgG and IgM, respectively. False-positive cases of IF-P and IP-P were 4, 5, and 11 and 5, 12, and 16 for IgA, IgG, and IgM, respectively.
Where IF-F lacks glomeruli or fresh renal biopsies are not available, IP-P is a sensitive method, whereas IF-P is a specific method for the evaluation of immune deposits in the renal tissue biopsies. The presence of false-positive cases in both methods deserves further research.
很少有已发表的研究比较在福尔马林固定、石蜡包埋（FFPE）组织切片上进行免疫荧光检测（IF-P）以及在FFPE组织切片上进行免疫过氧化物酶检测（IP-P）与在冰冻切片上进行免疫荧光检测（IF-F）用于评估肾脏疾病的情况。此外，每种方法的准确性差异很大。本研究的目的是评估IF-P和IP-P作为IF-F（金标准方法）的替代方法用于诊断肾活检标本的效果。
总共对101例肾活检标本进行IF-P、IP-P和IF-F染色，以显示免疫球蛋白IgA、IgG和IgM免疫沉积物。计算敏感性、特异性、假阳性和假阴性值。
IP-P对IgA、IgG和IgM显示的敏感性分别为61.8%、74.2%和64.2%以及特异性分别为84.8%、69.2%和66.7%。IF-P对IgA、IgG和IgM显示的敏感性分别为45.6%、69.4%和52.8%以及特异性分别为87.9%、87.2%和77.1%。IF-P和IP-P的IgA、IgG和IgM假阳性病例分别为4、5和11以及5、12和16。
在IF-F缺乏肾小球或无法获得新鲜肾活检标本的情况下，IP-P是一种敏感的方法，而IF-P是评估肾组织活检中免疫沉积物的一种特异性方法。两种方法中均存在假阳性病例，值得进一步研究。
