过滤对血浆中凝血因子残留水平的影响。

The effect of filtration on residual levels of coagulation factors in plasma.

机构信息

Blood Bank and Transfusion Medicine Research Unit, The Miriam Hospital, Providence, RI 02906, USA.

出版信息

Am J Clin Pathol. 2013 Jan;139(1):110-6. doi: 10.1309/AJCPRRESG7PGIAH5.

Abstract

Leukoreduced blood components are commonly manufactured via filtration. There are specifications for the residual leukocyte content of any final cellular blood component but not for residual clotting factors. Leukoreduced and nonleukoreduced platelet-poor plasma products were manufactured from filtered vs unfiltered platelet-rich plasma, respectively, using platelet leukoreduction filters. The leukoreduced plasma showed lower levels of factor VIII (75% ± 16% vs 88% ± 18%, P ≤ .05), factor XI (86% ± 9% vs 96% ± 10%, P ≤ .01) and factor VII (87% ± 14% vs 98% ± 11%, P ≤ .01). No difference was seen with factor X, factor V, or fibrinogen. Plasma filtered through a whole blood filter showed a reduction in factor V (105% ± 12% vs 124% ± 10%, P ≤ .01) but a minimal reduction in factor VIII (80% ± 5% vs 82% ± 6%, P = .04). Filtration can alter the residual levels of clotting factors to a variable extent in manufactured plasma, most noticeably factors V, VII, VIII, and XI.

摘要

经过滤处理通常可制备去白细胞血液成分。对于任何最终的细胞血液成分，都有关于残留白细胞含量的规定，但对于残留凝血因子并无相关规定。使用血小板去白细胞滤器，分别从过滤和未过滤的富含血小板血浆中制备去白细胞和非去白细胞血小板贫浆产品。去白细胞血浆中因子 VIII（75%±16%比 88%±18%，P≤.05）、因子 XI（86%±9%比 96%±10%，P≤.01）和因子 VII（87%±14%比 98%±11%，P≤.01）的水平较低。因子 X、因子 V 和纤维蛋白原则无差异。全血过滤器过滤的血浆中因子 V 减少（105%±12%比 124%±10%，P≤.01），但因子 VIII 减少幅度较小（80%±5%比 82%±6%，P=.04）。过滤可在一定程度上改变所制备血浆中凝血因子的残留水平，尤以因子 V、VII、VIII 和 XI 较为显著。