College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jan 15;913-914:61-8. doi: 10.1016/j.jchromb.2012.11.018. Epub 2012 Nov 29.
Molecularly imprinted anion-functionalized poly(ionic liquid)s (MAPILs) were prepared by radical polymerization for the multi-phase dispersive extraction (MPDE) of flavonoids from plants. Poly(ionic liquid)s were functionalized with different anions via anion metathesis to enhance their separation efficiency, called anion-functionalized poly(ionic liquid)s (APILs). A molecularly imprinting technique was introduced to produce specific recognition sites by forming complexes between the template molecules and anion-functionalized ionic liquid monomers to reduce the interactions with the interference substances and increase the selectivity. Multi-phase dispersive extraction (MPDE) was applied for separation instead of the traditional solid phase extraction method. The target compounds were first extracted by three-phase (sample-solvent-sorbent) dispersive extraction and cleaned up after removing the sample matrix. This method significantly decrease in the interference and analysis cost. A suitable sorbent for MPDE could be identified based on the adsorption behaviors of flavonoids on different MAPILs. The mean recovery yields of quercitrin, myricetin, and amentoflavone from Chamaecyparis obtusa under the optimized conditions were 88.07, 93.59, and 95.13%. This is a promising method for the extraction, separation and determination of flavonoids or other polyphenolic compounds from natural and other sources.
通过自由基聚合制备了分子印迹阴离子功能化聚（离子液体）（MAPILs），用于植物中黄酮类化合物的多相分散萃取（MPDE）。通过阴离子交换反应将聚离子液体功能化，引入不同的阴离子，以提高它们的分离效率，称为阴离子功能化聚（离子液体）（APILs）。通过模板分子与阴离子功能化离子液体单体之间形成配合物，引入分子印迹技术，产生特定的识别位点，以减少与干扰物质的相互作用，提高选择性。多相分散萃取（MPDE）被应用于分离，而不是传统的固相萃取方法。目标化合物首先通过三相（样品-溶剂-吸附剂）分散萃取进行提取，然后在去除样品基质后进行净化。这种方法显著减少了干扰和分析成本。可以根据黄酮类化合物在不同 MAPILs 上的吸附行为来确定适用于 MPDE 的合适吸附剂。在优化条件下，从扁柏中提取槲皮苷、杨梅素和穗花杉双黄酮的平均回收率分别为 88.07%、93.59%和 95.13%。这是一种从天然和其他来源中提取、分离和测定黄酮类化合物或其他多酚类化合物的有前途的方法。
