Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
Bioresour Technol. 2013 Mar;132:391-4. doi: 10.1016/j.biortech.2012.11.067. Epub 2012 Nov 29.
A novel and simple methodology for co-obtaining of enantiomerically pure α-hydroxyacids and α-ketoacids was developed by enantioselective oxidation of α-hydroxyacids bearing a substituent with an aryl group using α-hydroxyacid dehydrogenase (α-HADH). A high-throughput method was firstly established for screening of enantioselective α-HADHs. Sinorhizobium sp. ZJB1 101 with high activity and excellent enantioselectivity of α-HADH for oxidation of α-hydroxyacids bearing a substituent with an aryl group was isolated and identified. This strain has potential for co-production of (R)-α-hydroxyacids and α-ketoacids in near theoretical yields, while no consecutive oxidation of α-ketoacids was observed. The green conversion system appears promising for potential applications in industry.
开发了一种新颖而简单的方法，通过使用α-羟基酸脱氢酶（α-HADH）对具有芳基取代基的α-羟基酸进行对映选择性氧化，共同获得对映体纯的α-羟基酸和α-酮酸。首次建立了高通量方法来筛选对映选择性α-HADH。分离并鉴定了具有高活性和对具有芳基取代基的α-羟基酸氧化的α-HADH 具有优异对映选择性的根瘤菌 sp. ZJB1 101。该菌株具有在近乎理论产率下共同生产（R）-α-羟基酸和α-酮酸的潜力，而没有观察到α-酮酸的连续氧化。该绿色转化体系有望在工业中得到广泛应用。
