Department of Biotechnology, Periyar Maniammai University, Periyar Nagar, Vallam, Thanjavur 613403, Tamil Nadu, India.
Fish Shellfish Immunol. 2013 Feb;34(2):660-6. doi: 10.1016/j.fsi.2012.12.008. Epub 2012 Dec 27.
The study evaluated the effects of dietary doses of Lactobacillus plantarum VSG3 on the growth performance, immunity, and disease resistance of Labeo rohita juveniles against Aeromonas hydrophila infection. Fish (mean body weight 60 g) were fed with diet containing 0 (control), 10(6), 10(8), 10(10) cfu g(-1)L. plantarum VSG3 for 60 days. Various growth and immune parameters were examined at 30 and 60 days of post-feeding. Fish were challenged with A. hydrophila 60 days post-feeding and mortalities were recorded over 10 days post-infection. Results showed that administration of VSG3 for 60 days had significant effects (P < 0.05) on the specific growth rate (SGR) and feed utilization efficiency of L. rohita. Dietary administration of L. plantarum VSG3 significantly increased the serum lysozyme and alternative complement pathway (ACP) activities, phagocytosis and respiratory burst activity in L. rohita throughout the experimental period. The highest superoxide dismutase (SOD) activity (P < 0.05) was observed in the fish group fed diet containing VSG3 at 10(8) cfu g(-1). The serum IgM levels were significantly higher in the experimental groups compared to the control group after 30 days of feeding; but, the result was opposite after 60 days of feeding. Further, fish fed the diet containing 10(8) cfu g(-1)L. plantarum VSG3 had significantly higher (P < 0.05) post-challenge survival rate (77.7%). These results collectively suggest that dietary supplementation of L. plantarum VSG3 at 10(8) cfu g(-1) to L. rohita is optimal for enhancing the growth, immunity, and disease resistance against A. hydrophila infection.
该研究评估了植物乳杆菌 VSG3 的膳食剂量对罗非鱼幼鱼生长性能、免疫和对嗜水气单胞菌感染的抗病性的影响。鱼（平均体重 60 克）用含有 0（对照）、10（6）、10（8）、10（10）cfu g（-1）L. 植物乳杆菌 VSG3 的饮食喂养 60 天。在喂养后 30 和 60 天检查了各种生长和免疫参数。在喂养后 60 天用嗜水气单胞菌对鱼进行攻毒，并在感染后 10 天记录死亡率。结果表明，VSG3 给药 60 天对罗非鱼特定生长率（SGR）和饲料利用效率有显著影响（P < 0.05）。在整个实验期间，日粮添加植物乳杆菌 VSG3 显著提高了罗非鱼的血清溶菌酶和替代补体途径（ACP）活性、吞噬作用和呼吸爆发活性。在含有 VSG3 的日粮喂养的鱼组中观察到最高的超氧化物歧化酶（SOD）活性（P < 0.05）。与对照组相比，实验组在喂养 30 天后血清 IgM 水平显著升高；但在喂养 60 天后结果相反。此外，饲喂含有 10（8）cfu g（-1）L. 植物乳杆菌 VSG3 的日粮的鱼具有显著更高的（P < 0.05）攻毒后存活率（77.7%）。这些结果表明，日粮添加 10（8）cfu g（-1）L. 植物乳杆菌 VSG3 对罗非鱼最佳，可增强生长、免疫和对嗜水气单胞菌感染的抗病性。
