Wang Zhi-Peng, Xu Hong-Mei, Wang Guang-Yuan, Chi Zhe, Chi Zhen-Ming
Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, China.
Biochim Biophys Acta. 2013 Apr;1831(4):675-82. doi: 10.1016/j.bbalip.2012.12.010. Epub 2012 Dec 26.
In this study, the MIG1 gene in the oleaginous yeast Yarrowia lipolytica ACA-DC 50109 (the parent yeast) was disrupted and the disruptant M25 obtained could grow in yeast nitrogen base-N5000 medium without uracil or the medium with 2-deoxy-D-glucose. It was found that the cells of the disruptant M25 had more lipid bodies than those of its parent yeast. The disruptant M25 contained 48.7% (w/w) of oil based on its cell weight while the parent yeast only contained 36.0% (w/w) of oil. Transcript levels of many genes relevant to lipid biosynthesis in the disruptant M25 were enhanced compared to those of the same genes in the parent yeast. However, transcript level of the MFE1 gene, one of the genes relevant to fatty acid degradation was reduced in the disruptant M25 compared to that of the same gene in the parent yeast. Such changes in gene expression profile may cause the increased lipid biosynthesis in the disruptant M25. Biosynthesis of C18:1 fatty acid in the disruptant M25 was greatly enhanced compared to that in the parent yeast.
在本研究中，对产油酵母解脂耶氏酵母ACA-DC 50109（亲本酵母）中的MIG1基因进行了破坏，获得的破坏株M25能够在不含尿嘧啶的酵母氮源-N5000培养基或含有2-脱氧-D-葡萄糖的培养基中生长。发现破坏株M25的细胞比其亲本酵母的细胞含有更多的脂质体。破坏株M25基于其细胞重量含有48.7%（w/w）的油脂，而亲本酵母仅含有36.0%（w/w）的油脂。与亲本酵母中相同基因的转录水平相比，破坏株M25中许多与脂质生物合成相关的基因的转录水平有所提高。然而，与亲本酵母中相同基因相比，破坏株M25中与脂肪酸降解相关的基因之一MFE1基因的转录水平降低。基因表达谱的这种变化可能导致破坏株M25中脂质生物合成增加。与亲本酵母相比，破坏株M25中C18:1脂肪酸的生物合成大大增强。
