Gálvez-López Didiana, Chávez-Meléndez Bianca, Vázquez-Ovando Alfredo, Rosas-Quijano Raymundo
Instituto de Biociencias, Universidad Autónoma de Chiapas, Tapachula, Chiapas, México.
Unidad Académica Multidisciplinaria, Universidad Autónoma de Tamaulipas, Reynosa, México.
Braz J Microbiol. 2019 Jan;50(1):23-31. doi: 10.1007/s42770-018-0004-7. Epub 2018 Nov 29.
The biotechnological potential of Yarrowia lipolytica, as a single cell oil-producing microorganism, is presented in this review. Although initially this yeast species was considered as a lipid-degrading, recently, it was reclassified as a lipid-producing microorganism, since it has been reported to be capable of accumulating diverse desirable fatty acids after metabolic pathway engineering. In the first part of the present document, a general revision of the oil metabolic pathways and the capacity of oil production in Y. lipolytica is presented. The single cell oil produced by these metabolic engineering strategies has been designed by optimization, introduction, or suppression of new pathways to increase yield on lipid production. Later on, the genetic regulation systems and the lipid composition generated by this yeast for industrial purposes are discussed. These lipids could be safely used in the chemical food and biofuel industries, due to their high proportion of oleic acid. This document emphasizes in the overviewing at Y. lipolytica as an ideal oil cell factory, and as an excellent model to produce single cell oil.
本文综述了解脂耶氏酵母作为一种产单细胞油微生物的生物技术潜力。尽管最初这种酵母被认为是一种脂质降解菌，但最近它被重新归类为产脂质微生物，因为据报道，经过代谢途径工程改造后，它能够积累多种所需脂肪酸。在本文的第一部分，对解脂耶氏酵母的油脂代谢途径和产油能力进行了全面综述。通过优化、引入或抑制新途径来提高脂质产量，从而设计出了由这些代谢工程策略生产的单细胞油。随后，讨论了该酵母用于工业目的的遗传调控系统和产生的脂质组成。由于其油酸比例高，这些脂质可安全用于化学食品和生物燃料行业。本文着重概述了解脂耶氏酵母作为理想的油脂细胞工厂以及生产单细胞油的优秀模型。
