Grönberg G, Lipniunas P, Lundgren T, Lindh F, Nilsson B
Department of Carbohydrate Chemistry, University of Lund, Sweden.
Arch Biochem Biophys. 1990 May 1;278(2):297-311. doi: 10.1016/0003-9861(90)90264-y.
Five disialylated oligosaccharides, of which three were new compounds, were isolated from pooled human milk. The isolation procedure included gel filtration and ion-exchange chromatography and the final purification was accomplished by HPLC using a mobile phase containing triethylamine as an ion-pairing reagent. Structural analysis was carried out by chemical analyses, fast atom bombardment mass spectrometry, and 500-MHz NMR spectroscopy. The following new structures were found: (formula; see text).
