免疫性不孕女性和男性血清中 Izumo 抗体的存在和发生率。

Presence and incidence of izumo antibodies in sera of immunoinfertile women and men.

机构信息

Reproductive Immunology and Molecular Biology Laboratory, Department of Obstetrics and Gynecology, West Virginia University, School of Medicine, Morgantown, WV 26506-9186, USA.

出版信息

Am J Reprod Immunol. 2013 Mar;69(3):256-63. doi: 10.1111/aji.12060. Epub 2012 Dec 28.

DOI:10.1111/aji.12060

PMID:23279005

Abstract

PROBLEM

Sperm antigens that are relevant to fertilization and fertility, and are involved in human immunoinfertility, are being investigated in several laboratories. Izumo is a sperm-specific protein that has shown to be essential for sperm-egg fusion. The present study was conducted to examine the expression of Izumo protein in human sperm. Also, the presence and incidence of Izumo antibodies in sera of immunoinfertile women and men were investigated.

METHOD OF STUDY

The expression of Izumo protein in human sperm was examined by the Western blot procedure. Sera from immunoinfertile women (n = 25) and fertile women (n = 23), as well as sera from immunoinfertile men (n = 20) and fertile men (n = 15), were collected and analyzed for immunoreactivity with Izumo peptides in the enzyme-linked immunosorbent assay (ELISA). Three epitopes of human Izumo, namely one epitope of Izumo1, and two epitopes of Izumo2 [Izumo2 (K-15), and Izumo2 (T-17)], respectively, were selected for the present study.

RESULTS

In the Western blot procedure, the Izumo1 antibody recognized a specific protein band of ~39 kDa in human sperm extract. In ELISA, 56% of the immunoinfertile female sera reacted positively (≧3 S.D. units) with Izumo1, 40% with Izumo2 (K-15), and 20% with Izumo2 (T-17) peptide. None of the sera (0%) from fertile women reacted positively (≦3 S.D. units) with any of the three Izumo peptides. None of the sera from immunoinfertile (0%) or fertile men (0%) reacted positively with any of the three Izumo peptides.

CONCLUSION

Our findings indicate that human sperm expresses Izumo protein, and the immunoinfertile female sera have circulating isoantibodies against this protein. This is the first study to our knowledge, which examined the presence and incidence of Izumo antibodies in female and male immunoinfertility. These findings may find clinical applications in specific diagnosis and treatment of infertility, and contraceptive vaccine development.

摘要

问题

与受精和生育有关的精子抗原，以及与人类免疫性不育有关的精子抗原，正在几家实验室进行研究。Izumo 是一种精子特异性蛋白，已被证明对精卵融合至关重要。本研究旨在检测 Izumo 蛋白在人精子中的表达。此外，还研究了免疫性不育女性和男性血清中 Izumo 抗体的存在和发生率。

方法

通过 Western blot 程序检测人精子中 Izumo 蛋白的表达。收集免疫性不育女性（n = 25）和正常生育女性（n = 23）、免疫性不育男性（n = 20）和正常生育男性（n = 15）的血清，并通过酶联免疫吸附试验（ELISA）分析其与 Izumo 肽的免疫反应性。本研究选择了人类 Izumo 的三个表位，即 Izumo1 的一个表位和 Izumo2 的两个表位[Izumo2（K-15）和 Izumo2（T-17）]。

结果

在 Western blot 程序中，Izumo1 抗体识别人精子提取物中约 39 kDa 的特异性蛋白带。在 ELISA 中，56%的免疫性不育女性血清与 Izumo1 阳性反应（≧3 S.D.单位），40%与 Izumo2（K-15）阳性反应，20%与 Izumo2（T-17）阳性反应。正常生育女性血清（0%）与三种 Izumo 肽均无阳性反应（≦3 S.D.单位）。免疫性不育男性（0%）或正常生育男性（0%）血清与三种 Izumo 肽均无阳性反应。