Naz R K, Gateva E, Morte C
Department of Obstetrics and Gynecology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Arch Androl. 1995 Nov-Dec;35(3):225-31. doi: 10.3109/01485019508987875.
Autoantigenicity of human sperm as related to immunoinfertility was investigated using the immunoprecipitation procedure. Sera from immunoinfertile men specifically immunoprecipitated six specific bands of 135, 95, and 65 kD and double bands of 47, 41, and 23 kD that were not recognized by the sera from fertile men. Of these six specific bands, three bands of 65-, 47-, and 23-kD molecular identities were recognized by > 60% of immunoinfertile sera, and 47 and 23 kD may correspond to the dimeric and monomeric forms of FA-1 antigen, respectively. Seminal plasma from immunoinfertile men specifically recognized three specific bands of 95, 37, and 16 kD; only the 95-kD band was also recognized with the immunoinfertile sera. These specific antigens may find applications in immunocontraception and immunoinfertility in humans.
采用免疫沉淀法研究了与免疫性不育相关的人类精子自身抗原性。免疫性不育男性的血清特异性免疫沉淀出135、95和65kD的六条特异性条带以及47、41和23kD的双条带，而生育男性的血清无法识别这些条带。在这六条特异性条带中，65、47和23kD分子身份的三条条带被60%以上的免疫性不育血清识别，47和23kD可能分别对应FA-1抗原的二聚体和单体形式。免疫性不育男性的精浆特异性识别95、37和16kD的三条特异性条带；只有95kD条带也能被免疫性不育血清识别。这些特异性抗原可能在人类免疫避孕和免疫性不育中得到应用。
